Nikitin V P, Kozyrev S A, Solntseva S V
P. K. Anokhin Research Institute of Normal Physiology, Moscow, Russia.
Bull Exp Biol Med. 2017 Jan;162(3):295-299. doi: 10.1007/s10517-017-3598-0. Epub 2017 Jan 13.
Specificity of behavioral and neuronal mechanisms of impairment of long-term memory reconsolidation was studied in edible snails trained for associative skill of rejection of two types of food: raw carrots (conditioned stimulus 1) and apple (conditioned stimulus 2). In 2 days after training, the snails received protein synthesis inhibitor cycloheximide and a reminder (conditioned stimulus 1 or 2). In 3 and 14 days after cycloheximide/reminder, we observed the absence of aversive responses to the conditioned stimulus used as the reminder and preserved responses to the conditioned stimulus not used as the reminder. Moreover, we observed specific suppression of synaptic responses of command neurons of snail defensive behavior induced by the conditioned stimulus used as the reminder after cycloheximide injection and preserved synaptic responses of neurons to the other conditioned stimulus. It was hypothesized that protein synthesis-dependent synapse-specific plasticity of command neurons can be a mechanism of selective preservation of conditioned food aversion memory in snails.
在经过训练以掌握拒绝两种食物（生胡萝卜，条件刺激1；苹果，条件刺激2）的联想技能的食用蜗牛中，研究了长期记忆再巩固受损的行为和神经元机制的特异性。训练后两天，蜗牛接受蛋白质合成抑制剂环己酰亚胺和一个提示（条件刺激1或2）。在注射环己酰亚胺/提示后的第3天和第14天，我们观察到对用作提示的条件刺激没有厌恶反应，而对未用作提示的条件刺激仍有反应。此外，我们观察到注射环己酰亚胺后，用作提示的条件刺激所诱导的蜗牛防御行为指令神经元的突触反应受到特异性抑制，而神经元对另一种条件刺激的突触反应则得以保留。据推测，指令神经元的蛋白质合成依赖性突触特异性可塑性可能是蜗牛中条件性食物厌恶记忆选择性保留的一种机制。
