Bumgardner J D, Lucas L C, Tilden A B
Department of Biomedical Engineering, University of Alabama, Birmingham.
J Biomed Mater Res. 1989 Oct;23(10):1103-14. doi: 10.1002/jbm.820231002.
The biocompatibility of three commercial copper-based dental casting alloys--Duracast MS, Goldent, and Trindium--three experimental copper alloys, and a control gold alloy, Modulay, were investigated. Trindium, Duracast MS, experimental alloys 1 and 2 are aluminum bronzes; Goldent is a hybrid aluminum-brass alloy; and experimental alloy #3 is a high zinc brass alloy. ASTM F813-83 Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices, a 3-day direct-contact cell culture regimen and atomic absorption spectroscopy were utilized for evaluating the biocompatibility of these alloys in both Waymouth's and RPMI 1640 complete media. Cellular proliferation assays, using 3H-thymidine uptake, were also conducted in Waymouth's media. In this investigation, only the experimental alloy #3 elicited alterations in morphology and viability of the fibroblast monolayer during the ASTM and 3-day culture tests in either media. Cell cultures exposed to experimental alloy #3 experienced copper concentrations greater than 16.0 ppm in Waymouth's and 10 ppm copper in RPMI 1640 media. Differences in the size of the cytotoxic zone around experimental alloy #3 were also observed, with the larger zone occurring in Waymouth's media. In contrast to the direct cell contact studies, all alloys caused decreases in 3H-thymidine uptake in Waymouth's media at much reduced metal ion concentrations as compared to the controls. Thus, adverse changes in DNA synthesis occurred at much lower copper and zinc concentrations than changes in morphology and viability. Consequently, the assessment of biocompatibility is dependent on the parameters evaluated, and several parameters must be analyzed before a material may be considered biocompatible.
研究了三种市售铜基牙科铸造合金（Duracast MS、Goldent和Trindium）、三种实验性铜合金以及一种对照金合金Modulay的生物相容性。Trindium、Duracast MS、实验合金1和2是铝青铜；Goldent是一种混合铝黄铜合金；实验合金#3是一种高锌黄铜合金。采用ASTM F813 - 83《医疗器械材料直接接触细胞培养评价标准实施规程》、3天直接接触细胞培养方案和原子吸收光谱法，在Waymouth's培养基和RPMI 1640完全培养基中评估这些合金的生物相容性。还在Waymouth's培养基中进行了使用3H - 胸腺嘧啶核苷摄取的细胞增殖试验。在本研究中，在ASTM和3天培养试验期间，无论是在哪种培养基中，只有实验合金#3引起了成纤维细胞单层形态和活力的改变。暴露于实验合金#3的细胞培养物在Waymouth's培养基中的铜浓度大于16.0 ppm，在RPMI 1640培养基中的铜浓度为10 ppm。还观察到实验合金#3周围细胞毒性区域大小的差异，在Waymouth's培养基中该区域更大。与直接细胞接触研究相反，与对照相比，所有合金在Waymouth's培养基中在金属离子浓度大幅降低的情况下均导致3H - 胸腺嘧啶核苷摄取减少。因此，与形态和活力变化相比，DNA合成的不利变化在铜和锌浓度低得多时就会发生。因此，生物相容性的评估取决于所评估的参数，在一种材料被认为具有生物相容性之前，必须分析几个参数。
