Haque N, Singh M, Hossain S A
Department of Physiology and Biochemistry, College of Veterinary Sciences and AH, SDAU, Banaskantha, Gujarat, India.
Dairy Cattle Physiology Division, Karnal, Haryana, India.
J Anim Physiol Anim Nutr (Berl). 2018 Feb;102(1):166-174. doi: 10.1111/jpn.12674. Epub 2017 Jan 13.
Comprehensive information on the role of β-casein and plasminogen-plasmin (PG-PL) system in milk secretion of Murrah buffaloes during winter season is lacking, although effects of cold stress can be ameliorated to an extent by altering microclimate at farm level. Hence, this study was aimed to determine the changes in productivity along with PG-PL system of milk, plasma hormones and metabolites of buffaloes during winter (December-January) season under two different management systems. Average minimum temperature and wind chill index during this season were 7.02 and 12.74 °C respectively. Buffaloes were divided in two groups of six animals each: control and treatment, where treatment group animals were placed in-house with floor bedding of paddy straw and the control group animals in loose housing system without straw bedding. Physiological responses were recorded, and milk and blood samples were collected at weekly intervals for six-week experimental period. Under in-house management system, buffaloes experienced better comfort by alleviating environmental stress as their physiological responses such as respiration rate and pulse rate were significantly reduced (p < 0.01) as compared to the control, which subsequently resulted higher milk yield by 9.92% (p < 0.05). Analysis of milk samples revealed higher concentration of plasminogen (10.6 vs. 8.05 μg/ml; p < 0.01) and β-casein (p < 0.05), and lower plasmin level (0.299 vs. 0.321 μg/ml; p < 0.05) in buffaloes under treatment group. It was also found that plasma cortisol, glucose and non-esterified fatty acids levels were higher (p < 0.01) in control group as compared to the treatment animals by 13.6%, 8.14% and 12.6% respectively. However, milk composition, growth hormone, epinephrine and norepinephrine level in plasma were similar in both the groups. Hence, it may be concluded that provision of in-house shelter management with floor bedding of paddy straw during winter was effective to minimize environmental stress and improved milk production through manipulation of PG-PL system in buffaloes.
尽管通过改变农场层面的小气候可以在一定程度上缓解冷应激的影响，但关于冬季穆拉水牛泌乳过程中β-酪蛋白和纤溶酶原-纤溶酶（PG-PL）系统作用的全面信息尚缺。因此，本研究旨在确定在两种不同管理系统下，冬季（12月至1月）水牛的生产力变化以及牛奶、血浆激素和代谢产物的PG-PL系统变化。该季节的平均最低温度和风寒指数分别为7.02℃和12.74℃。水牛被分为两组，每组六头：对照组和处理组，处理组动物被安置在有稻草垫料的室内，对照组动物则饲养在没有稻草垫料的开放式畜舍系统中。记录生理反应，并在为期六周的实验期内每周采集牛奶和血液样本。在室内管理系统下，水牛通过减轻环境应激获得了更好的舒适度，因为与对照组相比，它们的呼吸频率和脉搏率等生理反应显著降低（p<0.01），随后产奶量提高了9.92%（p<0.05）。牛奶样本分析显示，处理组水牛的纤溶酶原浓度更高（10.6对8.05μg/ml；p<0.01）和β-酪蛋白浓度更高（p<0.05），而纤溶酶水平更低（0.299对0.321μg/ml；p<0.05）。还发现，对照组的血浆皮质醇、葡萄糖和非酯化脂肪酸水平比处理组动物分别高13.6%、8.14%和12.6%（p<0.01）。然而，两组的牛奶成分、生长激素、肾上腺素和去甲肾上腺素水平相似。因此，可以得出结论，冬季提供有稻草垫料的室内庇护所管理有效地减少了环境应激，并通过操纵水牛的PG-PL系统提高了牛奶产量。
