Disney J E, Johnson K R, Magnuson N S, Sylvester S R, Reeves R
Program in Genetics and Cell Biology, Washington State University, Pullman 99164-4660.
J Cell Biol. 1989 Nov;109(5):1975-82. doi: 10.1083/jcb.109.5.1975.
Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone "High-Mobility Group" protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes.
经短暂蛋白酶消化后，用吉姆萨染料染色，哺乳动物中期染色体可通过其特征性的带型进行识别。已知由此产生的G带含有富含A/T残基的DNA区域，并且是人类染色体中长散在重复序列L1（或Kpn 1）家族的主要定位区域。在此我们报告，针对一种高度纯化且生化特性明确的非组蛋白“高迁移率族”蛋白HMG-I产生的抗体，可将该蛋白特异性地定位到哺乳动物中期染色体的G带上。在一些染色体高度浓缩的标本中，HMG-I似乎也位于哺乳动物染色体的着丝粒和/或端粒区域。据我们所知，这是第一种主要定位于染色体G带区域的特性明确的哺乳动物蛋白。
