高迁移率族蛋白HMG-I定位于人类和小鼠染色体的G/Q带和C带。
High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.
作者信息
Disney J E, Johnson K R, Magnuson N S, Sylvester S R, Reeves R
机构信息
Program in Genetics and Cell Biology, Washington State University, Pullman 99164-4660.
出版信息
J Cell Biol. 1989 Nov;109(5):1975-82. doi: 10.1083/jcb.109.5.1975.
Abstract
Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone "High-Mobility Group" protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes.
摘要
经短暂蛋白酶消化后，用吉姆萨染料染色，哺乳动物中期染色体可通过其特征性的带型进行识别。已知由此产生的G带含有富含A/T残基的DNA区域，并且是人类染色体中长散在重复序列L1（或Kpn 1）家族的主要定位区域。在此我们报告，针对一种高度纯化且生化特性明确的非组蛋白“高迁移率族”蛋白HMG-I产生的抗体，可将该蛋白特异性地定位到哺乳动物中期染色体的G带上。在一些染色体高度浓缩的标本中，HMG-I似乎也位于哺乳动物染色体的着丝粒和/或端粒区域。据我们所知，这是第一种主要定位于染色体G带区域的特性明确的哺乳动物蛋白。
相似文献
1
High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes.高迁移率族蛋白HMG-I定位于人类和小鼠染色体的G/Q带和C带。
J Cell Biol. 1989 Nov;109(5):1975-82. doi: 10.1083/jcb.109.5.1975.
2
Metaphase chromosome structure: bands arise from a differential folding path of the highly AT-rich scaffold.中期染色体结构：带纹源于富含AT的支架的差异折叠路径。
Cell. 1994 Feb 25;76(4):609-22. doi: 10.1016/0092-8674(94)90502-9.
3
R-banding of human chromosomes by heat denaturation and Giemsa staining after amethopterin-synchronization.氨甲蝶呤同步化后经热变性和吉姆萨染色对人类染色体进行R显带。
Can J Genet Cytol. 1983 Jun;25(3):261-9. doi: 10.1139/g83-041.
4
Delineation of human prometaphase paracentromeric regions using sequential GTG- and C-banding.
Cytogenet Cell Genet. 1987;45(3-4):132-40. doi: 10.1159/000132444.
5
Restriction enzyme banding of mouse metaphase chromosomes.小鼠中期染色体的限制性酶切显带
Chromosoma. 1984;90(2):128-32. doi: 10.1007/BF00292449.
6
Spatial organization of large-scale chromatin domains in the nucleus: a magnified view of single chromosome territories.细胞核中大规模染色质结构域的空间组织：单个染色体区域的放大视图。
J Cell Biol. 1997 Dec 29;139(7):1597-610. doi: 10.1083/jcb.139.7.1597.
7
A view of interphase chromosomes.间期染色体的视图。
Science. 1990 Dec 14;250(4987):1533-40. doi: 10.1126/science.2274784.
8
Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.小鼠基因组中长短散在元件序列的差异分布：荧光原位杂交染色体核型分析
Proc Natl Acad Sci U S A. 1990 Oct;87(19):7757-61. doi: 10.1073/pnas.87.19.7757.
9
In situ factors affecting stability of the DNA helix in interphase nuclei and metaphase chromosomes.影响间期细胞核和中期染色体中DNA螺旋稳定性的原位因素。
Exp Cell Res. 1987 Sep;172(1):168-79. doi: 10.1016/0014-4827(87)90103-0.
10
Do individual allocyclic chromosomes in metaphase reflect their interphase domains?中期的单个异周染色体是否反映了它们的间期结构域？
Hum Genet. 1987 Nov;77(3):210-3. doi: 10.1007/BF00284471.
引用本文的文献
1
HMGA2 directly mediates chromatin condensation in association with neuronal fate regulation.HMGA2 直接介导与神经元命运调节相关的染色质凝聚。
Nat Commun. 2023 Oct 12;14(1):6420. doi: 10.1038/s41467-023-42094-9.
2
Nuclear High Mobility Group A2 (HMGA2) Interactome Revealed by Biotin Proximity Labeling.核高迁移率族 A2（HMGA2）相互作用组通过生物素邻近标记法揭示。
Int J Mol Sci. 2023 Feb 20;24(4):4246. doi: 10.3390/ijms24044246.
3
HMGA2 drives the IGFBP1/AKT pathway to counteract the increase in P27KIP1 protein levels in mtDNA/RNA-less cancer cells.HMGA2 驱动 IGFBP1/AKT 通路，以抵消 mtDNA/RNA 缺失癌细胞中 P27KIP1 蛋白水平的增加。
Cancer Sci. 2023 Jan;114(1):152-163. doi: 10.1111/cas.15582. Epub 2022 Sep 26.
4
The Linker Histone GH1-HMGA1 Is Involved in Telomere Stability and DNA Damage Repair.连接组蛋白 GH1-HMGA1 参与端粒稳定性和 DNA 损伤修复。
Plant Physiol. 2018 May;177(1):311-327. doi: 10.1104/pp.17.01789. Epub 2018 Apr 5.
5
Binding of high mobility group A proteins to the mammalian genome occurs as a function of AT-content.高迁移率族A蛋白与哺乳动物基因组的结合是根据AT含量而发生的。
PLoS Genet. 2017 Dec 21;13(12):e1007102. doi: 10.1371/journal.pgen.1007102. eCollection 2017 Dec.
6
High Mobility Group A2 protects cancer cells against telomere dysfunction.高迁移率族蛋白A2保护癌细胞免受端粒功能障碍的影响。
Oncotarget. 2016 Mar 15;7(11):12761-82. doi: 10.18632/oncotarget.6938.
7
Mechanisms of therapeutic resistance in cancer (stem) cells with emphasis on thyroid cancer cells.癌症（干细胞）中的治疗耐药机制，重点关注甲状腺癌细胞。
Front Endocrinol (Lausanne). 2014 Mar 25;5:37. doi: 10.3389/fendo.2014.00037. eCollection 2014.
8
Molecular and cellular characterization of an AT-hook protein from Leishmania.利什曼原虫 AT 钩蛋白的分子和细胞特征。
PLoS One. 2011;6(6):e21412. doi: 10.1371/journal.pone.0021412. Epub 2011 Jun 23.
9
Cross-linking of DNA through HMGA1 suggests a DNA scaffold.通过 HMGA1 交联 DNA 表明存在 DNA 支架。
Nucleic Acids Res. 2011 Sep 1;39(16):7124-33. doi: 10.1093/nar/gkr396. Epub 2011 May 19.
10
Nuclear functions of the HMG proteins.高迁移率族蛋白的核功能。
Biochim Biophys Acta. 2010 Jan-Feb;1799(1-2):3-14. doi: 10.1016/j.bbagrm.2009.09.001. Epub 2009 Sep 11.
本文引用的文献
1
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.荧光显微镜检查：没食子酸正丙酯减少罗丹明和荧光素蛋白缀合物的光漂白
Science. 1982 Sep 24;217(4566):1252-5. doi: 10.1126/science.7112126.
2
The nature and mechanisms of chromosome banding.染色体显带的本质与机制。
Cancer Genet Cytogenet. 1982 May;6(1):59-87. doi: 10.1016/0165-4608(82)90022-x.
3
Protein D1 preferentially binds A + T-rich DNA in vitro and is a component of Drosophila melanogaster nucleosomes containing A + T-rich satellite DNA.蛋白质D1在体外优先结合富含A+T的DNA，并且是含有富含A+T卫星DNA的黑腹果蝇核小体的一个组成部分。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7152-6. doi: 10.1073/pnas.79.23.7152.
4
Isolation, properties and cellular distribution of D1, a chromosomal protein of Drosophila.果蝇染色体蛋白D1的分离、特性及细胞分布
Chromosoma. 1980;78(1):1-31. doi: 10.1007/BF00291907.
5
Chromatin structure and gene activity: the role of nonhistone chromosomal proteins.染色质结构与基因活性：非组蛋白染色体蛋白的作用
CRC Crit Rev Biochem. 1982;13(1):1-86. doi: 10.3109/10409238209108709.
6
A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.一种蛋白质在三个特定位点与卫星DNA重复序列结合，这些位点在核小体中会通过DNA折叠而相互靠近。
Cell. 1984 Jul;37(3):889-901. doi: 10.1016/0092-8674(84)90424-0.
7
Transcriptionally active chromatin.转录活性染色质
Biochim Biophys Acta. 1984 Sep 10;782(4):343-93. doi: 10.1016/0167-4781(84)90044-7.
8
On the presence of two new high mobility group-like proteins in HeLa S3 cells.关于海拉S3细胞中两种新型高迁移率族样蛋白的存在。
FEBS Lett. 1983 Feb 21;152(2):163-7. doi: 10.1016/0014-5793(83)80370-6.
9
Electron microscopic visualisation of chromosomes banded with trypsin.
Nature. 1974 Feb 1;247(5439):292-4. doi: 10.1038/247292a0.
10
Production of G and C banding with progressive trypsin treatment.采用逐步胰蛋白酶处理进行G带和C带染色体制备。
Pediatr Res. 1973 Jan;7(1):39-44. doi: 10.1203/00006450-197301000-00006.
Zotero 插件