Nishi H, Tsumagari N, Kakimoto T, Terabe S
Analytical Chemistry Research Laboratory, Tanabe Seiyaku, Co., Ltd., Osaka, Japan.
J Chromatogr. 1989 Aug 30;477(2):259-70. doi: 10.1016/s0021-9673(01)89635-7.
The retention behaviour of beta-lactam antibiotics in micellar electrokinetic chromatography (EKC) was investigated. Sodium dodecyl sulphate (SDS) and sodium N-lauroyl-N-methyltaurate were used an anionic surfactants at concentrations of 0.05-0.3 M. It was found that the retention of ionic substances in micellar EKC is determined by the following three factors: the electrophoretic migration of the ionic substances, the interaction between the ionic substances and ionic surfactants and solubilization of the solute by the micellar phase. A difference in the retention behaviours of cationic substances was observed between the two anionic surfactants, which have different groups neighbouring the charge-bearing groups. The effect of an ion-pairing reagent was also investigated to make the effect of the micelle clearer. All test solutes were successfully separated by micellar EKC at SDS concentrations above 0.1 M, with theoretical plate numbers ranging from 70,000 to 260,000.
研究了β-内酰胺类抗生素在胶束电动色谱（EKC）中的保留行为。使用十二烷基硫酸钠（SDS）和N-月桂酰-N-甲基牛磺酸钠作为阴离子表面活性剂，浓度为0.05 - 0.3 M。发现在胶束EKC中离子物质的保留由以下三个因素决定：离子物质的电泳迁移、离子物质与离子表面活性剂之间的相互作用以及胶束相对溶质的增溶作用。在两种阴离子表面活性剂之间观察到阳离子物质保留行为的差异，这两种表面活性剂在带电荷基团附近具有不同的基团。还研究了离子对试剂的作用以使胶束的作用更清晰。在SDS浓度高于0.1 M时，所有测试溶质均通过胶束EKC成功分离，理论塔板数范围为70,000至260,000。
