Nishi H, Fukuyama T, Matsuo M, Terabe S
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Company, Ltd., Osaka, Japan.
J Pharm Sci. 1990 Jun;79(6):519-23. doi: 10.1002/jps.2600790613.
Using micellar electrokinetic chromatography (micellar EKC or MEKC) the retention behavior of twelve active ingredients used in cold medicines was investigated. The role of five different anionic surfactants was investigated by MEKC and the results were compared with those obtained by conventional capillary zone electrophoresis (CZE). The relative retention order of the 12 ingredients was significantly different among the five surfactants; the different elution orders were ascribed to the differences in the hydrophilic groups of the surfactants. The effects of surfactant concentration and buffer pH were also investigated. The ingredients were successfully separated within 30 min by micellar EKC with high theoretical plate numbers (approximately 200,000), and selectivity was much improved in comparison with CZE. Micellar EKC was applied by the internal standard method to the quantitation of some active ingredients combined in commercial preparations.
采用胶束电动色谱法（胶束EKC或MEKC）研究了感冒药中12种活性成分的保留行为。通过MEKC研究了5种不同阴离子表面活性剂的作用，并将结果与传统毛细管区带电泳（CZE）得到的结果进行了比较。5种表面活性剂中12种成分的相对保留顺序有显著差异；不同的洗脱顺序归因于表面活性剂亲水基团的差异。还研究了表面活性剂浓度和缓冲液pH值的影响。通过胶束EKC在30分钟内成功分离了这些成分，理论塔板数高（约200,000），与CZE相比选择性有了很大提高。胶束EKC采用内标法用于商业制剂中某些活性成分的定量分析。
