Nakaš Enita, Lauc Tomislav, Tiro Alisa, Džemidžić Vildana, Zukanović Amila, Franić Miljenko, Ivković Vladimir
Department of Orthodontics, School of Dentistry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Bosn J Basic Med Sci. 2017 Feb 21;17(1):23-28. doi: 10.17305/bjbms.2017.1715.
Orthodontic tooth movement is the result of bone remodeling that occurs in periodontal ligament and alveolar bone tissue as a response to mechanical loading of the tooth. The aim of this study is to investigate the time- and dose-response effects of locally administered clodronate on tooth movement. Sixty Wistar rats were randomly assigned to 4 groups of 15 specimens: E1 - application of 10 mMol of clodronate in 3-day intervals; E2 - application of 2.5 mMol of clodronate in 3-day intervals; E3 - application of 10 mMol of clodronate in 7-day intervals; E4 - application of 2.5 mMol of clodronate in 7-day intervals. A 50 μL clodronate solution was injected into a subperiosteal area to the right maxillary incisor. The left maxillary incisor served as a control, with an injection of saline solution. In 3-day interval application regime, there was no effect of clodronate dosing on tooth movement. In 7-day interval application regime, decreased tooth movement was observed with 10 mMol compared with 2.5 mMol clodronate concentration. However, decreased tooth movement was also observed when 2.5 mMol of clodronate was applied in 7-versus 3-day intervals. Conversely, no difference was observed when 10 mMol concentration was applied in 3- versus 7-day intervals. When clodronate is applied subperiosteally in the root area, it decreases the tooth movement. Tooth movement is impeded by the higher clodronate dosing, as well as by shorter application interval even with lower dosing. The purpose of future trials should, therefore, be to determine a safe therapeutic dose/interval application of clodronate in humans and their potential side effects.
正畸牙齿移动是牙周膜和牙槽骨组织中发生的骨重塑的结果，这种骨重塑是对牙齿机械负荷的一种反应。本研究的目的是调查局部应用氯膦酸盐对牙齿移动的时间和剂量反应效应。将60只Wistar大鼠随机分为4组，每组15个样本：E1组——每3天应用10 mmol氯膦酸盐；E2组——每3天应用2.5 mmol氯膦酸盐；E3组——每7天应用10 mmol氯膦酸盐；E4组——每7天应用2.5 mmol氯膦酸盐。将50 μL氯膦酸盐溶液注射到右上颌切牙的骨膜下区域。左上颌切牙作为对照，注射生理盐水。在每3天一次的应用方案中，氯膦酸盐给药对牙齿移动没有影响。在每7天一次的应用方案中，与2.5 mmol氯膦酸盐浓度相比，10 mmol时观察到牙齿移动减少。然而，当每7天应用2.5 mmol氯膦酸盐时，与每3天应用相比，也观察到牙齿移动减少。相反，当应用10 mmol浓度时，则未观察到每3天和每7天应用之间的差异。当在牙根区域骨膜下应用氯膦酸盐时，它会减少牙齿移动。较高的氯膦酸盐剂量以及较短的应用间隔（即使剂量较低）都会阻碍牙齿移动。因此，未来试验的目的应该是确定氯膦酸盐在人类中的安全治疗剂量/间隔应用及其潜在的副作用。
