Cao Xiang, Tang Zhiyuan, Huang Fang, Jin Qin, Zhou Xiaoyu, Shi Jiahai
Department of Cardiothoracic Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
Department of Respiratory Medicine, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
Oncotarget. 2017 Feb 21;8(8):12812-12819. doi: 10.18632/oncotarget.14559.
TMPRSS11D (HAT) belongs to the large type II transmembrane serine protease (TTSP) family, participating in various biological and physiological processes. TMPRSS11D expression has been reported during squamous cell carcinogenesis, however, its expression during non-small cell lung cancer (NSCLC) development has not been studied. In this study, we determined the mRNA and protein expression of TMPRSS11D in NSCLC tumorous and matched adjacent normal tissues by quantitative reverse transcription PCR (qRT-PCR) and tissue microarray immunohistochemistry analysis (TMA-IHC) respectively. TMPRSS11D protein expression in tumorous tissues were correlated with NSCLC patients' clinical characteristics and overall survival. Both TMPRSS11D mRNA and protein expression levels were significantly higher in NSCLC tumorous tissues than in adjacent normal tissues. High TMPRSS11D protein expression was associated with high TNM stages, and high TMPRSS11D protein expression is an independent prognostic marker in NSCLC. Based on our results, we conclude that TMPRSS11D could play a role in NSCLC development and progression. Because of its role in proteolysis of extracellular matrix, targeting TMPRSS11D may prevent the development of metastasis in NSCLC.
TMPRSS11D（HAT）属于大型II型跨膜丝氨酸蛋白酶（TTSP）家族，参与各种生物和生理过程。已有报道称TMPRSS11D在鳞状细胞癌发生过程中表达，然而，其在非小细胞肺癌（NSCLC）发展过程中的表达尚未得到研究。在本研究中，我们分别通过定量逆转录PCR（qRT-PCR）和组织芯片免疫组化分析（TMA-IHC）测定了NSCLC肿瘤组织及配对的相邻正常组织中TMPRSS11D的mRNA和蛋白表达。肿瘤组织中TMPRSS11D蛋白表达与NSCLC患者的临床特征和总生存期相关。NSCLC肿瘤组织中TMPRSS11D的mRNA和蛋白表达水平均显著高于相邻正常组织。TMPRSS11D蛋白高表达与高TNM分期相关，且TMPRSS11D蛋白高表达是NSCLC的独立预后标志物。基于我们的结果，我们得出结论，TMPRSS11D可能在NSCLC的发展和进展中发挥作用。由于其在细胞外基质蛋白水解中的作用，靶向TMPRSS11D可能预防NSCLC转移的发生。
