Chen Z R, Bochner F, Somogyi A
Department of Clinical and Experimental Pharmacology, University of Adelaide, Australia.
J Chromatogr. 1989 Jul 21;491(2):367-78. doi: 10.1016/s0378-4347(00)82854-6.
A novel high-performance liquid chromatographic method for the determination of codeine, norcodeine and morphine in plasma and urine has been developed. The compounds were separated on a cyano column (15 cm x 4.6 mm, 5 microns particle size) using a mobile phase of acetonitrile-triethylamine-distilled water (4:0.1:95.9, v/v) pH 3.1 and then determined by fluorescence detection. Calibration curves in the range 5-200 ng/ml for plasma and 0.1-10 micrograms/ml for urine were linear and passed through the origin. The imprecision and inaccuracy of the assay were less than 10% and the limits of detection were 2 ng/ml for all three compounds in human plasma.
已开发出一种用于测定血浆和尿液中可待因、去甲可待因和吗啡的新型高效液相色谱法。这些化合物在氰基柱（15 cm×4.6 mm，粒径5微米）上分离，使用乙腈 - 三乙胺 - 蒸馏水（4:0.1:95.9，v/v）pH 3.1的流动相，然后通过荧光检测进行测定。血浆中5 - 200 ng/ml以及尿液中0.1 - 10微克/ml范围内的校准曲线呈线性且通过原点。该测定法的不精密度和不准确度小于10%，人血浆中所有三种化合物的检测限均为2 ng/ml。
