Nardos Aschalew, Makonnen Eyasu
Pharmacology Unit, School of Medicine, Hawassa University, P O Box 1560, Hawassa, Ethiopia.
Department of Pharmacology, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia.
Malar J. 2017 Jan 13;16(1):25. doi: 10.1186/s12936-017-1677-3.
Malaria is one of the most life-threatening health problems worldwide and treatment has been compromised by drug resistance. Identifying lead molecules from natural products might help to find better anti-malarial drugs, since those obtained from natural sources are still effective against malarial parasites. This study aimed at investigating the in vivo antiplasmodial activity of crude extract of the leaves of Ajuga remota together with its safety in mice models.
In vivo parasite growth inhibitory effect of crude extract was assessed in mice inoculated with Plasmodium berghei (ANKA strain). The in vivo antiplasmodial activity of the test extract was performed against early infection (4-day suppressive test), curative effect against established infection and prophylactic effect against residual infection. Acute and sub-acute toxicity were carried out according to OECD guidelines.
In vivo parasite growth inhibition effect of hydroethanolic crude extract of A. remota was evaluated at 30, 50 and 100 mg/kg dose levels. It suppressed parasitaemia by 77.34% at 100 mg/kg dose level in the 4-day test. In curative and prophylactic potential tests, it suppressed parasitaemia by 66.67 and 59.66% at 100 mg/kg dose level, respectively. In vivo toxicity tests revealed no toxicity. All parasitaemia suppressions were statistically significant at P < 0.05 as compared to the vehicle-treated group. The crude extract also prolonged survival time in a dose dependent manner.
The investigation results suggest that the leave extract of Ajuga remota possesses antimalarial activity.
疟疾是全球最危及生命的健康问题之一，耐药性使治疗受到影响。从天然产物中鉴定先导分子可能有助于找到更好的抗疟药物，因为从天然来源获得的那些物质对疟原虫仍然有效。本研究旨在调查紫背金盘叶粗提物在小鼠模型中的体内抗疟活性及其安全性。
在接种伯氏疟原虫（ANKA株）的小鼠中评估粗提物的体内寄生虫生长抑制作用。对试验提取物的体内抗疟活性进行早期感染（4天抑制试验）、对已建立感染的治疗效果和对残余感染的预防效果研究。按照经合组织指南进行急性和亚急性毒性试验。
在30、50和100mg/kg剂量水平评估了紫背金盘水乙醇粗提物的体内寄生虫生长抑制作用。在4天试验中，100mg/kg剂量水平时其抑制疟原虫血症的比例为77.34%。在治疗和预防潜力试验中，100mg/kg剂量水平时其分别抑制疟原虫血症66.67%和59.66%。体内毒性试验未显示毒性。与赋形剂处理组相比，所有疟原虫血症抑制在P<0.05时具有统计学意义。粗提物还以剂量依赖方式延长了存活时间。
研究结果表明紫背金盘叶提取物具有抗疟活性。
