Bueno-Junior Lezio S, Ruggiero Rafael N, Rossignoli Matheus T, Del Bel Elaine A, Leite Joao P, Uchitel Osvaldo D
Department of Neuroscience and Behavioral Sciences, Ribeirao Preto Medical School University of Sao Paulo, Ribeirao Preto, Brazil.
Department of Morphology, Physiology and Stomatology, Dentistry School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil.
Physiol Rep. 2017 Jan;5(1). doi: 10.14814/phy2.13066. Epub 2017 Jan 13.
The knowledge on real-time neurophysiological effects of acetazolamide is still far behind the wide clinical use of this drug. Acetazolamide - a carbonic anhydrase inhibitor - has been shown to affect the neuromuscular transmission, implying a pH-mediated influence on the central synaptic transmission. To start filling such a gap, we chose a central substrate: hippocampal-prefrontal cortical projections; and a synaptic phenomenon: paired-pulse facilitation (a form of synaptic plasticity) to probe this drug's effects on interareal brain communication in chronically implanted rats. We observed that systemic acetazolamide potentiates the hippocampal-prefrontal paired-pulse facilitation. In addition to this field electrophysiology data, we found that acetazolamide exerts a net inhibitory effect on prefrontal cortical single-unit firing. We propose that systemic acetazolamide reduces the basal neuronal activity of the prefrontal cortex, whereas increasing the afferent drive it receives from the hippocampus. In addition to being relevant to the clinical and side effects of acetazolamide, these results suggest that exogenous pH regulation can have diverse impacts on afferent signaling across the neocortex.
关于乙酰唑胺实时神经生理效应的知识仍远远落后于该药物广泛的临床应用。乙酰唑胺——一种碳酸酐酶抑制剂——已被证明会影响神经肌肉传递，这意味着其对中枢突触传递存在pH介导的影响。为了开始填补这一空白，我们选择了一个中枢底物：海马-前额叶皮质投射；以及一种突触现象：双脉冲易化（一种突触可塑性形式），来探究该药物对慢性植入大鼠区域间脑通讯的影响。我们观察到，全身性给予乙酰唑胺会增强海马-前额叶双脉冲易化。除了这些场电生理学数据外，我们还发现乙酰唑胺对前额叶皮质单单位放电具有净抑制作用。我们提出，全身性给予乙酰唑胺会降低前额叶皮质的基础神经元活动，同时增加其从海马接收的传入驱动。除了与乙酰唑胺的临床应用和副作用相关外，这些结果还表明，外源性pH调节可对新皮质的传入信号产生多种影响。
