Salum M L, Giudicessi S L, Schmidt De León T, Camperi S A, Erra-Balsells R
CIHDECAR-CONICET y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, 3 piso, Ciudad Universitaria, 1428, Buenos Aires, Argentina.
Consejo Nacional de lnvestigaciones Cientificas y Técnicas, Instituto de Nanobiotecnología (NANOBIOTEC), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina.
J Mass Spectrom. 2017 Mar;52(3):182-186. doi: 10.1002/jms.3908.
Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd.
自从引入芥子酸（SA）和α-氰基-4-羟基肉桂酸作为基质以来，基质辅助激光解吸/电离质谱在蛋白质/多肽分析中开始成功应用。由于基质簇相当丰富，两者在短肽分析中都存在一些局限性。目前使用的肉桂酸是反式肉桂酸。在此，研究了Z-SA作为肽的基质，并与E-SA和α-氰基-4-羟基肉桂酸进行了比较。在低m/z区域总是观察到少量的簇，从而能够检测短肽。此处所述结果表明，这种新型基质是直接、快速和灵敏检测亲水性和疏水性肽的首选工具。版权所有© 2017约翰威立父子有限公司。
