Jiang Xiu-Qing, Cao Chang-Yu, Li Zhao-Yang, Li Wei, Zhang Cong, Lin Jia, Li Xue-Nan, Li Jing-Long
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, People's Republic of China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, People's Republic of China.
J Inorg Biochem. 2017 Apr;169:13-22. doi: 10.1016/j.jinorgbio.2017.01.002. Epub 2017 Jan 4.
Selenium (Se) incorporated in selenoproteins as selenocysteine and supports various important cellular and organismal functions. We recently reported that chicken brain exhibited high priority for Se supply and retention under conditions of dietary Se deficiency and supernutrition Li et al. (2012) . However, the selenotranscriptome expressions and their response to Se status in chicken central nervous system (CNS) are unclear. To better understand the relationship of Se homeostasis and selenoproteins expression in chicken CNS, 1day-old HyLine White chickens were fed a low Se diet (Se-L, 0.028mg/g) supplemented with 4 levels of dietary Se (0 to 5.0mgSe/kg) as NaSeO for 8weeks. Then chickens were dissected for getting the CNS, which included cerebral cortex, cerebellum, thalamus, bulbus cinereus and marrow. The expressions of selenoproteome which have 24 selenoproteins were detected by the quantitative real-time PCR array. The concept of a selenoprotein hierarchy was developed and the hierarchy of different regions in chicken CNS was existence, especially cerebral cortex and bulbus cinereus. The expression of selenoproteins has a hierarch while changing Se content, and Selenoprotein T (Selt), Selenoprotein K (Selk), Selenoprotein W (Selw), Selenoprotein U (Selu), Glutathione peroxidase 3 (Gpx3), Glutathione peroxidase 4 (Gpx4), Selenoprotein P (Sepp1), Selenoprotein O (Selo), Selenoprotein 15 (Sel15), Selenoprotein N (Seln), Glutathione peroxidase 2 (Gpx2) and Selenoprotein P 2 (Sepp2) take more necessary function in the chicken CNS. Therefore, we hypothesize that hierarchy of regulated the transcriptions of selenoproteome makes an important role of CNS Se metabolism and transport in birds.
作为硒代半胱氨酸掺入硒蛋白中的硒（Se）支持各种重要的细胞和机体功能。我们最近报道，在膳食硒缺乏和超营养条件下，鸡脑对硒的供应和保留具有高度优先性（Li等人，2012年）。然而，鸡中枢神经系统（CNS）中的硒转录组表达及其对硒状态的反应尚不清楚。为了更好地理解鸡中枢神经系统中硒稳态与硒蛋白表达的关系，将1日龄的海兰白鸡饲喂低硒日粮（Se-L，0.028mg/g），并添加4种水平的日粮硒（0至5.0mgSe/kg）作为亚硒酸钠，持续8周。然后解剖鸡以获取中枢神经系统，包括大脑皮层、小脑、丘脑、延髓和骨髓。通过定量实时PCR阵列检测具有24种硒蛋白的硒蛋白组的表达。提出了硒蛋白层次结构的概念，并且鸡中枢神经系统不同区域存在层次结构，尤其是大脑皮层和延髓。在改变硒含量时，硒蛋白的表达具有层次性，并且硒蛋白T（Selt）、硒蛋白K（Selk）、硒蛋白W（Selw）、硒蛋白U（Selu）、谷胱甘肽过氧化物酶3（Gpx3）、谷胱甘肽过氧化物酶4（Gpx4）、硒蛋白P（Sepp1）、硒蛋白O（Selo）、硒蛋白15（Sel15）、硒蛋白N（Seln）、谷胱甘肽过氧化物酶2（Gpx2）和硒蛋白P 2（Sepp2）在鸡中枢神经系统中发挥更重要的作用。因此，我们假设硒蛋白组转录调控的层次结构在鸟类中枢神经系统的硒代谢和转运中起重要作用。
