Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
J Photochem Photobiol B. 2017 Feb;167:216-227. doi: 10.1016/j.jphotobiol.2017.01.006. Epub 2017 Jan 7.
Lignans and neolignans are principal bioactive components of Linum usitatissimum L. (Flax), having multiple pharmacological activities. In present study, we are reporting an authoritative abiotic elicitation strategy of photoperiod regimes along with UV-C radiations. Cell cultures were grown in different photoperiod regimes (24h-dark, 24h-light and 16L/8D h photoperiod) either alone or in combination with various doses (1.8-10.8kJ/m) of ultraviolet-C (UV-C) radiations. Secoisolariciresinol diglucoside (SDG), lariciresinol diglucoside (LDG), dehydrodiconiferyl alcohol glucoside (DCG), and guaiacylglycerol-β-coniferyl alcohol ether glucoside (GGCG) were quantified by using reverse phase-high performance liquid chromatography (RP-HPLC). Results showed that the cultures exposed to UV-C radiations, accumulated higher levels of lignans, neolignans and other biochemical markers than cultures grown under different photoperiod regimes. 3.6kJ/m dose of UV-C radiations resulted in 1.86-fold (7.1mg/g DW) increase in accumulation of SDG, 2.25-fold (21.6mg/g DW) in LDG, and 1.33-fold (9.2mg/g DW) in GGCG in cell cultures grown under UV+photoperiod than their respective controls. Furthermore, cell cultures grown under UV+dark showed 1.36-fold (60.0mg/g DW) increase in accumulation of DCG in response to 1.8kJ/m dose of UV-C radiations. Smilar trends were observed in productivity of SDG, LDG and GGCG. Additionally, 3.6kJ/m dose of UV-C radiations also resulted in 2.82-fold (195.65mg/l) increase in total phenolic production, 2.94-fold (98.9mg/l) in total flavonoid production and 1.04-fold (95%) in antioxidant activity of cell cultures grown under UV+photoperiod. These findings open new dimensions for feasible production of biologically active lignans and neolignans by Flax cell cultures.
木脂素和新木脂素是亚麻（Linum usitatissimum L.）的主要生物活性成分，具有多种药理活性。在本研究中，我们报告了一种权威的非生物诱导策略，即光周期制度与 UV-C 辐射相结合。细胞培养物在不同的光周期制度（24h-黑暗、24h-光照和 16L/8D h 光周期）下单独或与不同剂量（1.8-10.8kJ/m）的紫外线-C（UV-C）辐射一起生长。通过反相高效液相色谱法（RP-HPLC）定量测定了开环异落叶松脂素二葡萄糖苷（SDG）、开环松脂素二葡萄糖苷（LDG）、去氢二松柏醇葡萄糖苷（DCG）和愈创木基甘油-β-松柏醇醚葡萄糖苷（GGCG）。结果表明，暴露于 UV-C 辐射的培养物比在不同光周期制度下生长的培养物积累了更高水平的木脂素、新木脂素和其他生化标志物。在 UV+光照下生长的细胞培养物中，3.6kJ/m 剂量的 UV-C 辐射导致 SDG 的积累增加了 1.86 倍（7.1mg/g DW），LDG 增加了 2.25 倍（21.6mg/g DW），GGCG 增加了 1.33 倍（9.2mg/g DW），而其各自的对照物则增加了 1.33 倍。此外，在 UV+黑暗下生长的细胞培养物对 1.8kJ/m 剂量的 UV-C 辐射的反应中，DCG 的积累增加了 1.36 倍（60.0mg/g DW）。SDG、LDG 和 GGCG 的产量也呈现出类似的趋势。此外，3.6kJ/m 剂量的 UV-C 辐射还导致 UV+光照下生长的细胞培养物的总酚产量增加了 2.82 倍（195.65mg/l），总类黄酮产量增加了 2.94 倍（98.9mg/l），抗氧化活性增加了 1.04 倍（95%）。这些发现为通过亚麻细胞培养物可行地生产具有生物活性的木脂素和新木脂素开辟了新的维度。
