胆固醇和麦角固醇会影响金黄色葡萄球菌抗生素外排泵的活性。
Cholesterol and ergosterol affect the activity of Staphylococcus aureus antibiotic efflux pumps.
作者信息
Tintino S R, Oliveira-Tintino C D M, Campina F F, Costa M S, Cruz R P, Pereira R L S, Andrade J C, Sousa E O, Siqueira-Junior J P, Coutinho H D M, Leal-Balbino T C, Balbino V Q
机构信息
Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry, CCBS, URCA, Brazil.
Laboratory of Microorganism Genetics (LGM), Department of Molecular Biology, CCEN, UFPB, Brazil.
出版信息
Microb Pathog. 2017 Mar;104:133-136. doi: 10.1016/j.micpath.2017.01.019. Epub 2017 Jan 11.
The aim of this study is to evaluate the effect of ergosterol on steroids and cholesterol efflux pumps in multidrug resistant strains of S. aureus. Were used RN4220 harboring plasmid pUL5054, which carries the gene encoding the MsrA macrolide efflux protein; and IS-58, which possesses the TetK tetracycline efflux protein; 1199B resists hydrophilic fluoroquinolones via a NorA-mediated mechanism and wild strain 1199B. The Minimal Inhibitory Concentration (MIC) was determined and the evaluation of possible inhibition of efflux pumps by reduction of MIC. Some of the detrimental effects on bacterial cells can be attributed to the detergent properties of cholesterol and ergosterol on account of their amphipathic structure. Besides the cholesterol did not affect directly the pump structure, a synergism was observed, maybe due the interaction with the cell membrane and interference in the lipid bilayer.
本研究的目的是评估麦角固醇对耐多药金黄色葡萄球菌中类固醇和胆固醇外排泵的影响。使用携带质粒pUL5054的RN4220，该质粒携带编码MsrA大环内酯外排蛋白的基因；以及具有TetK四环素外排蛋白的IS-58；1199B通过NorA介导的机制对亲水性氟喹诺酮耐药，还有野生菌株1199B。测定了最低抑菌浓度（MIC），并通过降低MIC来评估外排泵可能受到的抑制。由于胆固醇和麦角固醇的两亲结构，它们对细菌细胞的一些有害作用可归因于其去污剂特性。此外，胆固醇并未直接影响泵的结构，但观察到了协同作用，这可能是由于其与细胞膜的相互作用以及对脂质双层的干扰。
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