School of Food Science and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Institute of Agro-Production Processing Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Sci Rep. 2021 May 25;11(1):10927. doi: 10.1038/s41598-021-90377-2.
Nitrogen source is required for the growth of Cordyceps cicadae and involved in the regulation of metabolite synthesis. In order to further investigate the regulatory effects of nitrogen sources on the ergosterol synthesis by C. cicadae. We first confirmed that urea could significantly increase the ergosterol synthesis. The transcriptome analysis showed that compared with biomass cultured in the control fermentation medium (CFM), 1340 differentially expressed genes (DEGs) were obtained by Gene Ontology (GO) annotation, and 312 DEGs were obtained by Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation from the biomass cultured in CFM + CO(NH). Urea up-regulated D-3-phosphoglycerate dehydrogenase gene transcription level and down-regulated enolase and L-serine/L-threonine ammonialyase gene transcription level, increased serine synthesis, allosterically activate pyruvate kinase, to promote the synthesis of pyruvate and CHCO ~ SCOA, the primer of ergosterol; Urea increase the genes transcription related with ergosterol synthesis by up-regulating the steroid regulatory element binding protein gene transcription levels. The transcriptome results were provided by those of qRT-PCR. Collectively, our finding provided valuable insights into the regulatory effect of nitrogen source on the ergosterol synthesis by C. cicadae.
氮源是蝉花生长所必需的，并且参与代谢产物合成的调控。为了进一步研究氮源对蝉花甾醇合成的调控作用。我们首先证实尿素可以显著增加麦角甾醇的合成。转录组分析表明，与对照发酵培养基（CFM）中培养的生物量相比，从 CFM+CO(NH)中培养的生物量通过基因本体（GO）注释获得了 1340 个差异表达基因（DEGs），通过京都基因与基因组百科全书（KEGG）注释获得了 312 个 DEGs。尿素上调 D-3-磷酸甘油酸脱氢酶基因转录水平，下调烯醇酶和 L-丝氨酸/L-苏氨酸氨裂解酶基因转录水平，增加丝氨酸合成，别构激活丙酮酸激酶，促进丙酮酸和 CHCO~SCOA 的合成，麦角甾醇的前体；尿素通过上调甾醇调节元件结合蛋白基因转录水平，增加与麦角甾醇合成相关的基因转录。转录组结果由 qRT-PCR 结果提供。总之，我们的发现为氮源对蝉花甾醇合成的调控作用提供了有价值的见解。
