De Las Heras-Gómez Ignacio, Medina Sonia, Casas-Pina Teresa, Marín-Soler Lidia, Tomás Anna, Martínez-Hernández Pedro, Oger Camille, Galano Jean-Marie, Durand Thierry, Jimeno Luisa, Llorente Santiago, Lozoya Elena, Ferreres Federico, Gil-Izquierdo Ángel
Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Ctra. Madrid-Cartagena, s/n, 30120, El Palmar, Murcia, Spain.
Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
Free Radic Biol Med. 2017 Mar;104:178-184. doi: 10.1016/j.freeradbiomed.2017.01.019. Epub 2017 Jan 12.
F-neuroprostanes, F-neuroprostanes, and F-dihomo-isoprostanes, metabolites of non-enzymatic lipid peroxidation of polyunsaturated fatty acids [docosahexaenoic acid, n-6 docosapentanoic acid, and adrenic acid respectively], have become important biomarkers for oxidative stress in several diseases like epilepsy and alzheimer. These biomarkers and the 15-F-isoprostane (also known as 8-iso-PGF) a F-isoprostane isomer measured as reference oxidative marker at systemic level, were analyzed by UHPLC-QqQ-MS/MS in the urine of 60 renal recipients from cadaveric donors of the Nephrology Unit of the University Hospital Virgen de la Arrixaca, at six different times during the first six months after renal transplantation, and were compared with a control group of 60 healthy subjects from the same hospital. A total of 11 metabolites were analyzed and different patterns were observed. A tendency to decrease was observed in three metabolites (4-epi-4-F- NeuroP, ent-7(RS)-7-F-dihomo-IsoP, and ent-7(S)-7-F-dihomo-IsoP) and in our reference oxidative marker (15-F-IsoP) when kidney function improved and the excretion of urine proteins decreased. These results suggest that these three biomarkers of oxidative stress could be useful to assess renal function in the postransplant phase. Unfortunately, little is known about this kind of biomarker in this cohort of patients, so further investigation would be required in the clinical field to clarify the relationship between oxidative stress and the graft function, as well as the usefulness of these biomarkers as rejection markers.
F-神经前列腺素、F-神经前列腺素和F-二高异前列腺素分别是多不饱和脂肪酸（二十二碳六烯酸、n-6 二十二碳五烯酸和肾上腺酸）非酶促脂质过氧化的代谢产物，已成为癫痫和阿尔茨海默病等多种疾病中氧化应激的重要生物标志物。这些生物标志物以及作为全身水平参考氧化标志物测量的F-异前列腺素异构体15-F-异前列腺素（也称为8-异-PGF），通过超高效液相色谱-三重四极杆串联质谱（UHPLC-QqQ-MS/MS）在比利亚德阿里克斯卡大学医院肾病科尸体供体的60例肾移植受者的尿液中进行分析，在肾移植后的前六个月中的六个不同时间点进行检测，并与来自同一医院的60名健康受试者组成的对照组进行比较。共分析了11种代谢产物，并观察到不同的模式。当肾功能改善且尿蛋白排泄减少时，观察到三种代谢产物（4-表-4-F-神经前列腺素、内消旋-7(RS)-7-F-二高异前列腺素和内消旋-7(S)-7-F-二高异前列腺素）以及我们的参考氧化标志物（15-F-异前列腺素）有下降趋势。这些结果表明，这三种氧化应激生物标志物可能有助于评估移植后阶段的肾功能。不幸的是，对于这类患者群体中的这种生物标志物知之甚少，因此需要在临床领域进行进一步研究，以阐明氧化应激与移植肾功能之间的关系，以及这些生物标志物作为排斥反应标志物的有用性。
