Liang Hongwei, Guo Shanshan, Luo Xiangzhong, Li Zhong, Zou Guiwei
Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, No. 8, 1st Wudayuan Road, Wuhan East-lake Hi-tech Development Zone, Wuhan, 430223 China ; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070 China.
Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, No. 8, 1st Wudayuan Road, Wuhan East-lake Hi-tech Development Zone, Wuhan, 430223 China.
Springerplus. 2016 Dec 30;5(1):2115. doi: 10.1186/s40064-016-3766-0. eCollection 2016.
Bagridae is an important family of catfishes and has a high market demand. Recently, more cultivable Bagridae fishes are being exploited in China, and hybridization of some species has been carried out to achieve better growth performance, favorable sex ratios and better disease resistance. Yet, these hybrids have further increased the difficulties of taxonomy identification due to morphological indistinguishableness.
In this study, the molecular identification technologies for , sand their hybrids were successfully established by using mitochondrial and nuclear ITS sequences to identify the maternal and paternal lineage, respectively.
These molecular diagnostic methods could also be used to manage breeding plans of hybrids, monitor and minimize the negative impacts of hybridization programs in aquaculture. Furthermore, our study could also provide a reference for establishing detection technique for hybrids in other groups of fishes.
鲿科是鲶鱼的一个重要科,市场需求很高。近年来,中国正在开发更多可养殖的鲿科鱼类,并对一些物种进行了杂交,以实现更好的生长性能、有利的性别比例和更强的抗病能力。然而,由于形态上难以区分,这些杂交种进一步增加了分类鉴定的难度。
在本研究中,通过分别使用线粒体和核ITS序列来鉴定母系和父系谱系,成功建立了 、 及其杂交种的分子鉴定技术。
这些分子诊断方法还可用于管理杂交种的育种计划,监测并尽量减少水产养殖中杂交计划的负面影响。此外,我们的研究还可为建立其他鱼类杂交种的检测技术提供参考。