Favrot Claude, Linek Monika, Fontaine Jacques, Beco Luc, Rostaher Ana, Fischer Nina, Couturier Nicolas, Jacquenet Sandrine, Bihain Bernard E
Clinic for Small Animal Internal Medicine, University of Zurich, Winterthurerstrasse 204, CH-8057, Zürich, Switzerland.
Tieraerztliche Spezialisten, Rodigallee 85, D-22043, Hamburg, Germany.
Vet Dermatol. 2017 Apr;28(2):189-e42. doi: 10.1111/vde.12412. Epub 2017 Jan 16.
Food allergy is often suspected in dogs with clinical signs of atopic dermatitis. This diagnosis is confirmed with an elimination diet and a subsequent challenge with regular food. Laboratory tests for the diagnosis of food allergy in dogs are unreliable and/or technically difficult. Cyno-DIAL is a Western blot method that might assist with the selection of an appropriate elimination diet.
HYPOTHESIS/OBJECTIVES: To evaluate the performance of Cyno-DIAL for the selection of an elimination diet and diagnosis of food allergy.
ANIMALS/METHODS: Thirty eight dogs with atopic dermatitis completed an elimination diet. Combining the results of the diet trials and the challenges, 14 dogs were classified as food allergic (FA), 22 as nonfood-allergic and two as ambiguous cases.
Amongst all dogs and amongst dogs with a clinical diagnosis of FA, 3% and 7% (respectively) were positive to Royal Canin Anallergenic , Vet-Concept Kanguru or Vet-Concept Dog Sana ; 8% and 7% to Hill's d/d Duck and Rice ; 8% and 21% to Hill's z/d Ultra Allergen Free ; 53% and 64% to Eukanuba Dermatosis FP ; and 32% and 43% to a home-cooked diet of horse meat, potatoes and zucchini. The specificity and sensitivity of Cyno-DIAL for diagnosing food allergy were 73% and 71%, respectively.
Although Cyno-DIAL was considered potentially useful for identifying appropriate foods for elimination diet trials, it cannot be recommended for the diagnosis of food allergy. The Cyno-DIAL test performed better than some previously evaluated ELISA-based tests.
患有特应性皮炎临床症状的犬只常被怀疑存在食物过敏。通过排除性饮食及随后用常规食物进行激发试验来确诊这一诊断。用于犬类食物过敏诊断的实验室检测不可靠且/或技术难度大。Cyno - DIAL是一种蛋白质印迹法，可能有助于选择合适的排除性饮食。
假设/目的：评估Cyno - DIAL在选择排除性饮食及诊断食物过敏方面的性能。
动物/方法：38只患有特应性皮炎的犬只完成了排除性饮食。综合饮食试验和激发试验的结果，14只犬被归类为食物过敏（FA），22只为非食物过敏，2只为不明确病例。
在所有犬只以及临床诊断为FA的犬只中，对皇家犬粮低敏粮、Vet - Concept Kanguru或Vet - Concept Dog Sana呈阳性的分别为3%和7%；对希尔斯鸭肉米饭低敏粮呈阳性的为8%和7%；对希尔斯z/d超低敏粮呈阳性的为8%和21%；对优卡皮肤健康粮呈阳性的为53%和64%；对由马肉、土豆和西葫芦组成的自制饮食呈阳性的为32%和43%。Cyno - DIAL诊断食物过敏的特异性和敏感性分别为73%和71%。
尽管Cyno - DIAL被认为可能有助于识别用于排除性饮食试验的合适食物，但不推荐将其用于食物过敏的诊断。Cyno - DIAL检测比一些先前评估的基于酶联免疫吸附测定（ELISA）的检测表现更好。
