Maina Elisa, Matricoti Irina, Noli Chiara
Laboratory of Immunology, Ghent University of Veterinary Medicine, Salisburylaan 133, Merelbeke, 9820, Belgium.
Servizi Dermatologici Veterinari, Via Santo Stefano 168, Bologna, 40125, Italy.
Vet Dermatol. 2018 Jun;29(3):217-e78. doi: 10.1111/vde.12536. Epub 2018 Apr 6.
Adverse food reaction (AFR) is diagnosed with a two month elimination diet (ED), followed by challenge with the original food.
HYPOTHESIS/OBJECTIVES: To evaluate reactivity of selected EDs and performance of a Western blot serological test for the diagnosis of AFR.
Twenty five food reactive (FR) and 13 non food reactive (NFR) privately owned dogs.
Sera were tested for antibodies against hydrolyzed poultry feather (RCA), hydrolyzed soy (PHA), hydrolyzed fish (FUH), limited antigen horse and potato (THP), fresh horse meat and the offending food for each FR dog as documented by provocative challenge.
Fourteen sera were negative and two positive to all foods. Sera from five of 13 NFR and three of 25 FR dogs were reactive to hydrolyzed foods. The RCA diet was recognized by four of 38, FUH by six of 38 and PHA by one of 28 samples. THP was recognized by 14 of 33 and fresh horse by one of ten dogs that had never eaten horse meat. The test correctly identified one of 15 dogs allergic to FUH. Twenty of 25 FR sera were negative for the dogs' respective offending foods (20% sensitivity), whereas four of 13 NFR sera were positive to the dogs' usual diets (69% specificity).
Western blot analysis cannot be considered as a valid tool for the diagnosis of AFR; it may serve as an aid in selecting an ED.
不良食物反应（AFR）通过为期两个月的排除饮食（ED）进行诊断，随后用原始食物进行激发试验。
假设/目的：评估所选排除饮食的反应性以及用于AFR诊断的蛋白质印迹血清学检测的性能。
25只食物反应性（FR）和13只非食物反应性（NFR）的私人饲养犬。
检测血清中针对水解家禽羽毛（RCA）、水解大豆（PHA）、水解鱼（FUH）、有限抗原马和马铃薯（THP）、新鲜马肉以及每只FR犬通过激发试验记录的可疑食物的抗体。
14份血清对所有食物呈阴性，2份呈阳性。13只NFR犬中的5只和25只FR犬中的3只的血清对水解食物有反应。38份样本中4份对RCA饮食有反应，6份对FUH有反应，28份样本中1份对PHA有反应。33份样本中14份对THP有反应，10只从未吃过马肉的犬中有1只对新鲜马肉有反应。该检测正确识别出15只对FUH过敏的犬中的1只。25份FR血清中有20份对犬各自的可疑食物呈阴性（敏感性为20%），而13份NFR血清中有4份对犬的常规饮食呈阳性（特异性为69%）。
蛋白质印迹分析不能被视为诊断AFR的有效工具；它可作为选择排除饮食的辅助手段。
