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来自意大利蜜蜂的碳酸酐酶：纯化及农药抑制作用

Carbonic anhydrase from Apis mellifera: purification and inhibition by pesticides.

作者信息

Soydan Ercan, Güler Ahmet, Bıyık Selim, Şentürk Murat, Supuran Claudiu T, Ekinci Deniz

机构信息

a Faculty of Agriculture, Department of Agricultural Biotechnology , Ondokuz Mayıs University , Samsun , Turkey.

b Faculty of Agriculture, Department of Animal Science , Ondokuz May?s University , Samsun , Turkey.

出版信息

J Enzyme Inhib Med Chem. 2017 Dec;32(1):47-50. doi: 10.1080/14756366.2016.1232255.

DOI:10.1080/14756366.2016.1232255
PMID:28090787
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6009862/
Abstract

Carbonic anhydrase (CA) enzymes have been shown to play an important role in ion transport and in pH regulation in several organisms. Despite this information and the wealth of knowledge regarding the significance of CA enzymes, few studies have been reported about bee CA enzymes and the hazardous effects of chemicals. Using Apis mellifera as a model, this study aimed to determine the risk of pesticides on Apis mellifera Carbonic anhydrase enzyme (Am CA). CA was initially purified from Apis mellifera spermatheca for the first time in the literature. The enzyme was purified with an overall purification of ∼35-fold with a molecular weight of ∼32 kDa. The enzyme was then exposed to pesticides, including tebuconazole, propoxur, carbaryl, carbofuran, simazine and atrazine. The six pesticides dose-dependently inhibited in vitro AmCA activity at low micromolar concentrations. IC values for the pesticides were 0.0030, 0.0321, 0.0031, 0.0087, 0.0273 and 0.0165 μM, respectively. The AmCA inhibition mechanism of these compounds is unknown at this moment.

摘要

碳酸酐酶（CA）已被证明在多种生物体的离子运输和pH调节中发挥重要作用。尽管有这些信息以及关于CA酶重要性的丰富知识，但关于蜜蜂CA酶和化学物质有害影响的研究报道却很少。本研究以意大利蜜蜂为模型，旨在确定农药对意大利蜜蜂碳酸酐酶（Am CA）的风险。在文献中，CA首次从意大利蜜蜂的受精囊中被纯化出来。该酶经纯化后总体纯化倍数约为35倍，分子量约为32 kDa。然后将该酶暴露于包括戊唑醇、残杀威、西维因、克百威、西玛津和莠去津在内的农药中。这六种农药在低微摩尔浓度下对体外AmCA活性呈剂量依赖性抑制。这些农药的半数抑制浓度（IC）值分别为0.0030、0.0321、0.0031、0.0087、0.0273和0.0165 μM。目前这些化合物对AmCA的抑制机制尚不清楚。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d56d/6009862/23a91f941f36/IENZ_A_1232255_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d56d/6009862/a6f90ab7e2cd/IENZ_A_1232255_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d56d/6009862/23a91f941f36/IENZ_A_1232255_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d56d/6009862/a6f90ab7e2cd/IENZ_A_1232255_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d56d/6009862/23a91f941f36/IENZ_A_1232255_F0002_C.jpg

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