Department of Environmental Engineering, Peking University, Beijing 100871, China.
The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
Sci Rep. 2017 Jan 16;7:39289. doi: 10.1038/srep39289.
Anammox is a cost-effective process to treat nitrogenous wastewater. In this work, excitation-emission matrix (EEM) fluorescence spectroscopy was used to characterize the intracellular and extracellular substances of anammox sludge during reactor operation of 276 days. Four main fluorophores were identified from the intracellular substances. Two main protein-like fluorophores were identified from the extracellular substances. Correlation analysis revealed that intracellular 420 peak and humic-like peak had strong correlation with nitrogen removal rate. The two intracellular protein-like peaks had high correlation with MLVSS and MLVSS growth rate. Correlation analysis between different fluorophores discovered that the two peaks in each of these three groups-two intracellular protein-like peaks, two humic acid-like peaks and the two extracellular protein-like peaks had strong intercorrelation, which gave evidence of their homology. A specific method for fluorescence monitoring of anammox reactor were put forward, which included typical fluorescence indexes and their possible values for different operation phases.
厌氧氨氧化是一种处理含氮废水的经济有效方法。在这项工作中，采用激发-发射矩阵（EEM）荧光光谱法对 276 天的厌氧氨氧化污泥反应器运行过程中的胞内和胞外物质进行了表征。从胞内物质中鉴定出了 4 种主要的荧光物质。从胞外物质中鉴定出了 2 种主要的蛋白质样荧光物质。相关分析表明，胞内 420 峰和腐殖质样峰与氮去除率具有很强的相关性。这两个胞内蛋白样峰与 MLVSS 和 MLVSS 增长率具有很高的相关性。不同荧光物质之间的相关分析发现，这三组中每组的两个峰——两个胞内蛋白样峰、两个腐殖酸样峰和两个胞外蛋白样峰之间具有很强的相关性，这证明了它们的同源性。提出了一种用于厌氧氨氧化反应器荧光监测的特定方法，包括不同运行阶段的典型荧光指标及其可能的值。
