Huculeci Radu, Kieken Fabien, Garcia-Pino Abel, Buts Lieven, van Nuland Nico, Lenaerts Tom
Jean Jeener NMR Center, Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium.
Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussel, Belgium.
Methods Mol Biol. 2017;1555:257-267. doi: 10.1007/978-1-4939-6762-9_14.
Src homology 2 (SH2) domains are key modulators in various signaling pathways allowing the recognition of phosphotyrosine sites of different proteins. Despite the fact that SH2 domains acquire their biological functions in a monomeric state, a multitude of reports have shown their tendency to dimerize. Here, we provide a technical description on how to isolate and characterize by gel filtration, circular dichroism (CD), and nuclear magnetic resonance (NMR) each conformational state of p59 SH2 domain.
Src同源2（SH2）结构域是各种信号通路中的关键调节因子，可识别不同蛋白质的磷酸酪氨酸位点。尽管SH2结构域在单体状态下发挥其生物学功能，但大量报道表明它们有二聚化的倾向。在此，我们提供了一份技术说明，介绍如何通过凝胶过滤、圆二色性（CD）和核磁共振（NMR）对p59 SH2结构域的每种构象状态进行分离和表征。
