Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Insect Sci. 2018 Jun;25(3):401-408. doi: 10.1111/1744-7917.12440. Epub 2017 Mar 20.
Nilaparvata lugens and Sogatella furcifera are two primary planthoppers on rice throughout Asian countries and areas. Neonicotinoid insecticides, such as imidacloprid (IMI), have been extensively used to control rice planthoppers and IMI resistance consequently occurred with an important mechanism from the over-expression of P450 genes. The induction of P450 genes by IMI may increase the ability to metabolize this insecticide in planthoppers and increase the resistance risk. In this study, the induction of P450 genes was compared in S. furcifera treated with IMI and nitromethyleneimidazole (NMI), in two planthopper species by IMI lethal dose that kills 85% of the population (LD ), and in N. lugens among three IMI doses (LD , LD and LD ). When IMI and NMI at the LD dose were applied to S. furcifera, the expression changes in most P450 genes were similar, including the up-regulation of nine genes and down-regulation of three genes. In terms of the expression changes in 12 homologous P450 genes between N. lugens and S. furcifera treated with IMI at the LD dose, 10 genes had very similar patterns, such as up-regulation in seven genes, down-regulation in one gene and no significant changes in two genes. When three different IMI doses were applied to N. lugens, the changes in P450 gene expression were much different, such as up-regulation in four genes at all doses and dose-dependent regulation of the other nine genes. For example, CYP6AY1 could be induced by all IMI doses, while CYP6ER1 was only up-regulated by the LD dose, although both genes were reported important in IMI resistance. In conclusion, P450 genes in two planthopper species showed similar regulation patterns in responding to IMI, and the two neonicotinoid insecticides had similar effects on P450 gene expression, although the regulation was often dose-dependent.
褐飞虱和白背飞虱是亚洲国家和地区水稻上的两种主要飞虱。新烟碱类杀虫剂，如吡虫啉（IMI），已被广泛用于防治稻飞虱，而 IMI 抗性的产生主要与 P450 基因的过度表达有关。IMI 诱导 P450 基因的表达可能会增加飞虱代谢这种杀虫剂的能力，并增加抗性风险。在这项研究中，我们比较了两种飞虱在 IMI 和硝基亚甲基咪唑（NMI）处理下 P450 基因的诱导情况，在两种飞虱中用 IMI 致死剂量（杀死 85%种群的剂量，LD85）处理，以及在褐飞虱中用三种 IMI 剂量（LD50、LD25 和 LD10）处理。当用 IMI 和 NMI 的 LD 剂量处理白背飞虱时，大多数 P450 基因的表达变化相似，包括 9 个基因上调和 3 个基因下调。在 LD 剂量的 IMI 处理下，褐飞虱和白背飞虱的 12 个同源 P450 基因的表达变化中，有 10 个基因的模式非常相似，如 7 个基因上调，1 个基因下调，2 个基因无显著变化。当用三种不同的 IMI 剂量处理褐飞虱时，P450 基因表达的变化差异很大，如在所有剂量下 4 个基因上调，以及其他 9 个基因的剂量依赖性调节。例如，CYP6AY1 可被所有 IMI 剂量诱导，而 CYP6ER1 仅被 LD 剂量上调，尽管这两个基因都被报道在 IMI 抗性中很重要。总之，两种飞虱的 P450 基因在对 IMI 的反应中表现出相似的调节模式，两种新烟碱类杀虫剂对 P450 基因表达有相似的影响，尽管调节通常是剂量依赖性的。
