Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Pestic Biochem Physiol. 2019 Feb;154:39-45. doi: 10.1016/j.pestbp.2018.12.008. Epub 2018 Dec 18.
The brown planthopper, Nilaparvata lugens (Stål), is one of the most economically important rice pests in Asia and has become resistant to various kinds of insecticides, including neonicotinoid insecticides. In this study, an N. lugens clothianidin-resistant (CLR) strain and a susceptible (CLS) strain were established, and the potential resistance mechanisms of N. lugens to clothianidin were elucidated. The cross-resistance studies showed that the clothianidin-resistant strain exhibited cross-resistance to most neonicotinoid insecticides, especially nitenpyram (99.19-fold) and dinotefuran (77.68-fold), while there was no cross-resistance to chlorpyrifos (1.79-fold). The synergism assays and the activities of the detoxification enzymes were performed, and we found that a cytochrome P450 conferred the clothianidin resistance. Two P450 genes (CYP6ER1 and CYP6AY1) were found to be significantly overexpressed in the CLR strain compared with the CLS strain based on qRT-PCR. In addition, the knockdown of CYP6ER1 by RNA interference dramatically increased the toxicity of clothianidin against N. lugens. These data demonstrated that the overexpression of CYP6ER1 could contribute to clothianidin resistance in N. lugens. Our findings will help to improve the design of effective resistance management strategies to control brown planthoppers.
褐飞虱，Nilaparvata lugens（Stål），是亚洲最重要的水稻害虫之一，已对包括新烟碱类杀虫剂在内的各种杀虫剂产生抗性。在本研究中，建立了褐飞虱对氯虫苯甲酰胺的抗性（CLR）品系和敏感（CLS）品系，并阐明了褐飞虱对氯虫苯甲酰胺的潜在抗性机制。抗药性研究表明，氯虫苯甲酰胺抗性品系对大多数新烟碱类杀虫剂表现出交叉抗性，尤其是吡虫啉（99.19 倍）和噻虫嗪（77.68 倍），而对毒死蜱没有交叉抗性（1.79 倍）。通过协同作用测定和解毒酶活性测定，发现一种细胞色素 P450 赋予了褐飞虱对氯虫苯甲酰胺的抗性。基于 qRT-PCR，发现与 CLS 品系相比，CLR 品系中两个 P450 基因（CYP6ER1 和 CYP6AY1）显著过表达。此外，通过 RNA 干扰敲低 CYP6ER1 可显著提高氯虫苯甲酰胺对褐飞虱的毒性。这些数据表明，CYP6ER1 的过表达可能导致褐飞虱对氯虫苯甲酰胺的抗性。我们的研究结果将有助于提高控制褐飞虱的有效抗性管理策略的设计。
