Jason J, Ou C Y, Moore J L, Lawrence D N, Schochetman G, Evatt B L
Center for Infectious Diseases, Atlanta, Georgia.
J Infect Dis. 1989 Nov;160(5):789-94. doi: 10.1093/infdis/160.5.789.
Polymerase chain reaction (PCR) was used to detect human immunodeficiency virus (HIV)-1 DNA in peripheral blood mononuclear cells to assess in hemophilic men whether any were HIV-seropositive but uninfected or seronegative but infected and in seronegative sex partners of seropositive hemophilic men whether any were infected. Of 40 seropositive men, 38 (95%) were PCR-positive; one was PCR-indeterminate and one PCR-negative. None of 41 seronegative men who used only donor-screened, virus-inactivated coagulation factor products were PCR-positive. However, two of six who received noninactivated products were PCR-positive; one had low T-helper cell counts and died of unrelated causes and the other had seroconverted 11 mo later. PCR with a second primer pair also detected HIV-1 DNA in these two men. None of 25 seronegative female sex partners of seropositive men, including six men with AIDS and seven with AIDS-related symptoms, were PCR-positive. These data suggest that most seropositive hemophilic men are HIV-infected; whether some are infected with defective virus remains to be resolved as does the infection status of seropositive PCR-negative men. Identification of two seronegative PCR-positive men supports the possibility that HIV-1 DNA can be detected before seroconversion.
采用聚合酶链反应（PCR）检测外周血单个核细胞中的人类免疫缺陷病毒1型（HIV-1）DNA，以评估血友病男性中是否存在HIV血清学阳性但未感染或血清学阴性但已感染的个体，以及血友病血清学阳性男性的血清学阴性性伴侣中是否存在感染者。在40名血清学阳性男性中，38名（95%）PCR检测呈阳性；1名结果不确定，1名PCR检测呈阴性。41名仅使用经过供体筛查且病毒灭活的凝血因子产品的血清学阴性男性中，无人PCR检测呈阳性。然而，在6名接受未灭活产品的男性中，有2名PCR检测呈阳性；其中1名T辅助细胞计数较低，死于无关原因，另1名在11个月后血清阳转。使用第二对引物进行的PCR也在这两名男性中检测到了HIV-1 DNA。在25名血清学阳性男性的血清学阴性女性性伴侣中，无人PCR检测呈阳性，其中包括6名患有艾滋病的男性和7名有艾滋病相关症状的男性。这些数据表明，大多数血清学阳性的血友病男性感染了HIV；一些人是否感染了缺陷病毒以及血清学阳性但PCR检测呈阴性的男性的感染状况仍有待确定。两名血清学阴性但PCR检测呈阳性的男性的发现支持了在血清阳转前可检测到HIV-1 DNA的可能性。
