低人类免疫缺陷病毒1型（HIV-1）DNA载量是临床标本中通过聚合酶链反应（PCR）未能检测到HIV-1 DNA的主要原因。
Low human immunodeficiency virus type 1 (HIV-1) DNA burden as a major cause for failure to detect HIV-1 DNA in clinical specimens by PCR.
作者信息
Zazzi M, Romano L, Catucci M, De Milito A, Almi P, Gonnelli A, Rubino M, Valensin P E
机构信息
Department of Molecular Biology, University of Siena, Italy.
出版信息
J Clin Microbiol. 1995 Jan;33(1):205-8. doi: 10.1128/jcm.33.1.205-208.1995.
Abstract
To determine the sensitivity of a nested PCR procedure for detecting human immunodeficiency virus type 1 DNA in clinical specimens, 553 peripheral blood mononuclear cell samples obtained from 268 human immunodeficiency virus type 1-seropositive subjects were assayed by use of two independent primer sets for each sample. Overall, 1,088 of 1,106 (98.37%) reactions were positive. Investigation of the negative reactions showed that a low viral burden in some infected subjects, rather than primer-template mismatches, was the primary cause for the false-negative PCR results.
摘要
为确定巢式聚合酶链反应（PCR）程序检测临床标本中1型人类免疫缺陷病毒（HIV-1）DNA的灵敏度，对从268名1型人类免疫缺陷病毒血清反应阳性受试者获取的553份外周血单个核细胞样本，每个样本使用两套独立引物进行检测。总体而言，1106次反应中有1088次（98.37%）呈阳性。对阴性反应的调查显示，部分感染受试者病毒载量低而非引物-模板错配是PCR结果假阴性的主要原因。
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