大鼠快速上颌扩弓过程中基质金属蛋白酶-2和基质金属蛋白酶组织抑制因子-1的表达
Expression of MMP-2 and TIMP-1 during rapid maxillary expansion in rats.
作者信息
Chen Jianwei, Zhou Jing, Li Fan, Sun Jianfeng, Li Guifeng, Zou Shujuan, Ye Qingsong
机构信息
The State Key Laboratory of Oral Diseases and Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Stomatological Department, Yan'an Hospital of Kunming City, Kunming, China.
出版信息
Arch Oral Biol. 2017 Apr;76:30-35. doi: 10.1016/j.archoralbio.2017.01.002. Epub 2017 Jan 4.
OBJECTIVE
The aim of this study was to investigate the expression of MMP-2 and TIMP-1 during midpalatal suture expansion in rats.
DESIGN
72 male Wistar rats were randomly divided into 2 groups: the experimental group and the control group. In the experimental group, opening loops were applied across the midpalatal suture with an initial force of 50g, whereas in the control group, rats were subjected to sham installation of opening loops without activation. On day 1, 4, 7 and 14, nine rats from each group were sacrificed, and the maxillae were dissected and prepared for Immunohistochemistry (IHC) and RT- PCR examination of MMP-2 and TIMP-1 expression.
RESULTS
The results of IHC and Real Time PCR revealed that both protein and mRNA expression of MMP-2 and TIMP-1 were significantly increased after midpalatal expansion, and the ratio of MMP-2/TIMP-1 was also significantly enhanced.
CONCLUSIONS
The data suggested that MMP-2 and TIMP-1 might play an important role during the mid-palatal suture remodeling process of maxillary expansion.
目的
本研究旨在探讨基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶组织抑制因子-1（TIMP-1）在大鼠腭中缝扩展过程中的表达情况。
设计
将72只雄性Wistar大鼠随机分为2组：实验组和对照组。实验组在腭中缝处施加初始力为50g的开环装置，而对照组的大鼠接受未激活的开环装置假安装。在第1、4、7和14天，每组处死9只大鼠，解剖上颌骨并准备进行MMP-2和TIMP-1表达的免疫组织化学（IHC）和逆转录聚合酶链反应（RT-PCR）检测。
结果
免疫组织化学和实时定量PCR结果显示，腭中缝扩展后MMP-2和TIMP-1的蛋白和mRNA表达均显著增加，且MMP-2/TIMP-1比值也显著升高。
结论
数据表明，MMP-2和TIMP-1可能在上颌扩展腭中缝重塑过程中起重要作用。
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