Soares Rogério Nogueira, Reimer Raylene A, Murias Juan Manuel
Faculty of Kinesiology, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4, Canada; Coordination for the Improvement of Higher Education Personnel (CAPES), Setor Bancario Norte, Quadra 2 Bloco L, Brasilia, DF 70.040-031, Brazil.
Faculty of Kinesiology, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4, Canada; Department of Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N, Canada.
Microvasc Res. 2017 May;111:67-71. doi: 10.1016/j.mvr.2017.01.003. Epub 2017 Jan 14.
Postprandial hyperglycemia is associated with impaired vascular responsiveness. Recent studies have established the use of a near-infrared spectroscopy (NIRS)-derived approach, combined with a vascular occlusion test (VOT), to examine vascular responsiveness within the microvasculature in normoglycemia. The aim of this study was to examine whether this NIRS-VOT technique could detect differences in vascular responsiveness following a hyperglycemic challenge.
Fourteen young healthy individuals participated in the study. Participants underwent five vascular occlusion tests (pre, 30, 60, 90 and 120min after glucose ingestion). Vascular responsiveness was determined by the slope 2 of oxygen saturation (Slope 2 StO) and the area under the curve of oxygen saturation (StO) signal. All individuals had a significant increase in blood glucose concentration after 30min (p<0.001), 60min (p<0.001) and 90min (p<0.01) after glucose ingestion. The Slope 2 StO increased at 90min (p<0.01) after glucose ingestion. Conversely, The StO was significantly decreased 90min following the glucose challenge (p<0.01). A negative correlation was observed between average StO and average Slope 2 StO (r=-0.995; p<0.05).
This study showed that the NIRS-VOT technique is capable of detecting differential changes in vascular responsiveness to a hyperglycemic challenge, as indicated by a steeper Slope 2 StO and decreased StO following 90min of an oral glucose challenge.
餐后高血糖与血管反应性受损有关。最近的研究已证实，使用基于近红外光谱（NIRS）的方法并结合血管闭塞试验（VOT），可在血糖正常情况下检测微血管内的血管反应性。本研究的目的是检验这种NIRS-VOT技术能否检测高血糖刺激后血管反应性的差异。
14名年轻健康个体参与了本研究。参与者接受了五次血管闭塞试验（葡萄糖摄入前、摄入后30、60、90和120分钟）。通过氧饱和度斜率2（Slope 2 StO）和氧饱和度（StO）信号曲线下面积来确定血管反应性。所有个体在葡萄糖摄入后30分钟（p<0.001）、60分钟（p<0.001）和90分钟（p<0.01）时血糖浓度均显著升高。葡萄糖摄入后90分钟时，Slope 2 StO升高（p<0.01）。相反，葡萄糖刺激后90分钟时，StO显著降低（p<0.01）。观察到平均StO与平均Slope 2 StO之间呈负相关（r=-0.995；p<0.05）。
本研究表明，NIRS-VOT技术能够检测血管对高血糖刺激反应性的差异变化，口服葡萄糖刺激90分钟后，Slope 2 StO变陡以及StO降低即表明了这一点。
