Faculty of Kinesiology, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1N4, Canada.
Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Via Felice Casorati, 43, 37131, Verona, VR, Italy.
Appl Physiol Nutr Metab. 2019 Oct;44(10):1105-1111. doi: 10.1139/apnm-2018-0866. Epub 2019 Feb 25.
This study investigated changes in muscle oxidative metabolism and microvascular responsiveness induced by glucose ingestion in the upper and lower limbs using near-infrared spectroscopy (NIRS). Fourteen individuals (aged 27 ± 1.4 years) underwent 5 vascular occlusion tests (VOT) (pre-intervention (Pre), 30 min, 60 min, 90 min, and 120 min after glucose challenge). NIRS-derived oxygen saturation (StO) was measured on the forearm and leg muscle at each VOT. Muscle oxidative metabolism was determined by the StO downslope during cuff inflation (deoxygenation slope); microvascular responsiveness was estimated by the StO upslope (reperfusion slope) following cuff deflation. There was a significant increase in arm ( < 0.05; 1-β = 0.860) and leg ( < 0.05; 1-β = 1.000) oxidative metabolism activity as represented by the faster deoxygenation slope at 60, 90, and 120 min (0.08 ± 0.03, 0.08 ± 0.03, 0.08 ± 0.02%·s, respectively) (leg) and at 90 min (0.16 ± 0.08%·s) (arm) observed after glucose ingestion when compared with their respective Pre values (leg = 0.06 ± 0.02; arm = 0.11 ± 0.04%·s). There was a significant increase in arm ( < 0.05; 1-β = 0.880) and leg ( < 0.05; 1-β = 0.983) reperfusion slope at 60 min (arm = 3.63 ± 2.1%·s; leg = 1.56 ± 0.6%·s), 90 min (arm = 3.91 ± 2.1%·s; leg = 1.60 ± 0.6%·s), and 120 min (arm = 3.91 ± 1.6%·s; leg = 1.54 ± 0.6%·s) when compared with their Pre values (arm = 2.79 ± 1.7%·s; leg = 1.26 ± 0.5%·s). Our findings showed that NIRS-VOT technique is capable of detecting postprandial changes in muscle oxidative metabolism activity and microvascular reactivity in the upper and lower limb. NIRS-VOT is a promising noninvasive clinical approach that may help in the early, limb-specific detection of impairments in glucose oxidation and microvascular function.
本研究使用近红外光谱（NIRS）技术探讨了葡萄糖摄入对上、下肢肌肉氧化代谢和微血管反应性的变化。14 名个体（年龄 27 ± 1.4 岁）接受了 5 次血管闭塞试验（VOT）（预干预（Pre）、30 分钟、60 分钟、90 分钟和 120 分钟后葡萄糖挑战）。在每次 VOT 时，通过 NIRS 测量前臂和腿部肌肉的氧饱和度（StO）。肌肉氧化代谢通过袖带充气时 StO 的下降斜率（脱氧斜率）来确定；微血管反应性通过袖带放气后的 StO 上升斜率（再灌注斜率）来估计。在 60、90 和 120 分钟时，手臂（<0.05；1-β=0.860）和腿部（<0.05；1-β=1.000）的氧化代谢活性均显著增加（<0.05；1-β=0.880），表现为再灌注斜率更快（手臂=0.08±0.03、0.08±0.03、0.08±0.02%·s，分别）（腿部），以及在 90 分钟时（手臂=0.16±0.08%·s）（手臂）葡萄糖摄入后观察到的更快的脱氧斜率（腿=0.06±0.02；手臂=0.11±0.04%·s）。与各自的 Pre 值相比，手臂（<0.05；1-β=0.880）和腿部（<0.05；1-β=0.983）的再灌注斜率在 60 分钟（手臂=3.63±2.1%·s；腿部=1.56±0.6%·s）、90 分钟（手臂=3.91±2.1%·s；腿部=1.60±0.6%·s）和 120 分钟（手臂=3.91±1.6%·s；腿部=1.54±0.6%·s）时均显著增加。我们的发现表明，NIRS-VOT 技术能够检测到上、下肢肌肉氧化代谢活性和微血管反应性的餐后变化。NIRS-VOT 是一种很有前途的非侵入性临床方法，可能有助于早期、特异性地检测葡萄糖氧化和微血管功能障碍。
