Li Yinghua, Li Meihua, Luo Honglin, Bai Jing, Zhang Jianquan, Zhong Xiaoning, Lan Xiuwan, He Zhiyi
Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
Guangxi Medical University, Nanning 530021, Guangxi, China.
Microb Pathog. 2017 Mar;104:155-160. doi: 10.1016/j.micpath.2017.01.025. Epub 2017 Jan 14.
Talaromyces marneffei is an important opportunistic pathogenic fungus capable of causing systemic lethal infection through inhalation of its conidia. However, little is known about the pathogenesis and interactions between Talaromyces marneffei and host. The aim of this study was to identify potential long noncoding RNAs (lncRNAs) and coding genes associated with interactions between airway epithelial cell and Talaromyces marneffei conidia. We carried out a microarray analysis to determine the expression profile of lncRNA and mRNA in human bronchial epithelial cell in response to Talaromyces marneffei infection. Compared to control group, we found that 370 and 149 lncRNAs were up and down regulated, respectively. Meanwhile, the expression level of 269 and 60 mRNAs was increased and decreased, respectively. To understand the potential role of the differentially expressed lncRNAs, we performed functional annotations of the corresponding coding genes using gene ontology and pathway analyses. Our results provide insights into the pathogenesis of early infection by Talaromyces marneffei.
马尔尼菲篮状菌是一种重要的机会致病性真菌，可通过吸入其分生孢子引起全身性致死性感染。然而，关于马尔尼菲篮状菌与宿主之间的发病机制和相互作用知之甚少。本研究的目的是鉴定与气道上皮细胞和马尔尼菲篮状菌分生孢子相互作用相关的潜在长链非编码RNA（lncRNA）和编码基因。我们进行了微阵列分析，以确定人支气管上皮细胞在马尔尼菲篮状菌感染后的lncRNA和mRNA表达谱。与对照组相比，我们发现分别有370个和149个lncRNA上调和下调。同时，269个和60个mRNA的表达水平分别升高和降低。为了了解差异表达lncRNA的潜在作用，我们使用基因本体论和通路分析对相应的编码基因进行了功能注释。我们的结果为马尔尼菲篮状菌早期感染的发病机制提供了见解。
