Juárez-Vega Guillermo, Rangel-Ramírez Velia, Monsiváis-Urenda Adriana, Niño-Moreno Perla, Garcia-Sepúlveda Christian, Noyola Daniel E, González-Amaro Roberto
Department of Immunology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico.
Department of Microbiology, School of Medicine, UASLP, San Luis Potosí, SLP, Mexico.
Hum Immunol. 2017 Mar;78(3):274-280. doi: 10.1016/j.humimm.2017.01.004. Epub 2017 Jan 16.
Human cytomegalovirus (HCMV) infection in children and young adults has been associated with changes in the innate immune system. We herein analyzed the possible effect of very long term HCMV infection on the expression of several NK cell receptors. Ninety HCMV-seropositive individuals were included and classified as young adults (n=30), elderly (n=30) and very elderly subjects (n=30). A peripheral blood sample was obtained and the expression of NK cell receptors (NKG2A, NKG2C, ILT2, CD161, KIR2DL1, KIR3DL1, and KIR3DL2) by NK and other lymphocyte subsets was assessed by flow cytometry. In addition, the frequency of the sixteen KIR genes was analyzed by polymerase chain reaction. We found a significant increase in the number of NKG2C+ NK and T cells in elderly individuals compared to young adults accompanied by an opposite trend in the number of NKG2A+ lymphocytes, and ILT2+ cells were also increased in elderly individuals. A significant increase in the levels of CD3-CD56+NKG2C+CD57+ cells was also detected in the elderly groups. Finally, KIR gene analysis revealed that the KIR genotype 2 was significantly less frequent in the elderly individuals. Our results support that long-term infection by HCMV exerts a significant progressive effect on the innate immune system.
儿童和青年人群中的人巨细胞病毒（HCMV）感染与先天性免疫系统的变化有关。我们在此分析了长期HCMV感染对几种自然杀伤（NK）细胞受体表达的可能影响。研究纳入了90名HCMV血清学阳性个体，并将其分为青年（n = 30）、老年人（n = 30）和高龄老人（n = 30）。采集外周血样本，通过流式细胞术评估NK细胞和其他淋巴细胞亚群中NK细胞受体（NKG2A、NKG2C、ILT2、CD161、KIR2DL1、KIR3DL1和KIR3DL2）的表达。此外，通过聚合酶链反应分析16种杀伤细胞免疫球蛋白样受体（KIR）基因的频率。我们发现，与青年相比，老年人中NKG2C + NK细胞和T细胞数量显著增加，同时NKG2A +淋巴细胞数量呈现相反趋势，并且老年人中ILT2 +细胞数量也增加。在老年组中还检测到CD3 - CD56 + NKG2C + CD57 +细胞水平显著升高。最后，KIR基因分析显示，KIR基因型2在老年人中的频率显著较低。我们的结果支持HCMV长期感染对先天性免疫系统具有显著的渐进性影响。
