Hsu Hwei-Jan, Drummond-Barbosa Daniela
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
Gene Expr Patterns. 2017 Jan;23-24:13-21. doi: 10.1016/j.gep.2017.01.001. Epub 2017 Jan 16.
The effect of diet on reproduction is well documented in a large number of organisms; however, much remains to be learned about the molecular mechanisms underlying this connection. The Drosophila ovary has a well described, fast and largely reversible response to diet. Ovarian stem cells and their progeny proliferate and grow faster on a yeast-rich diet than on a yeast-free (poor) diet, and death of early germline cysts, degeneration of early vitellogenic follicles and partial block in ovulation further contribute to the ∼60-fold decrease in egg laying observed on a poor diet. Multiple diet-dependent factors, including insulin-like peptides, the steroid ecdysone, the nutrient sensor Target of Rapamycin, AMP-dependent kinase, and adipocyte factors mediate this complex response. Here, we describe the results of a visual screen using a collection of green fluorescent protein (GFP) protein trap lines to identify additional factors potentially involved in this response. In each GFP protein trap line, an artificial GFP exon is fused in frame to an endogenous protein, such that the GFP fusion pattern parallels the levels and subcellular localization of the corresponding native protein. We identified 53 GFP-tagged proteins that exhibit changes in levels and/or subcellular localization in the ovary at 12-16 hours after switching females from rich to poor diets, suggesting them as potential candidates for future functional studies.
饮食对繁殖的影响在大量生物体中已有充分记录；然而，关于这种联系背后的分子机制仍有许多有待了解。果蝇卵巢对饮食有明确、快速且在很大程度上可逆的反应。与无酵母（贫乏）饮食相比，富含酵母的饮食能使卵巢干细胞及其后代增殖和生长得更快，早期生殖系囊肿的死亡、早期卵黄生成卵泡的退化以及排卵的部分阻滞进一步导致了在贫乏饮食条件下观察到的产卵量约60倍的下降。多种饮食依赖性因子，包括胰岛素样肽、类固醇蜕皮激素、营养传感器雷帕霉素靶蛋白、AMP依赖激酶和脂肪细胞因子介导了这种复杂反应。在此，我们描述了一项视觉筛选的结果，该筛选使用了一组绿色荧光蛋白（GFP）蛋白陷阱品系来鉴定可能参与这种反应的其他因子。在每个GFP蛋白陷阱品系中，一个人工GFP外显子与一个内源性蛋白读码框融合，使得GFP融合模式与相应天然蛋白的水平和亚细胞定位平行。我们鉴定出53种带有GFP标签的蛋白，它们在雌性果蝇从丰富饮食转换为贫乏饮食后12 - 16小时，在卵巢中的水平和/或亚细胞定位出现变化，这表明它们是未来功能研究的潜在候选对象。
