Sanchez Leonardo R, Breser Maria L, Godoy Gloria J, Salazar Florencia C, Mackern-Oberti Juan P, Cuffini Cecilia, Motrich Ruben D, Rivero Virginia E
Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI)-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Instituto de Medicina y Biología Experimental de Cuyo (IMBECU-CCT Mendoza, CONICET), Mendoza, Argentina.
Prostate. 2017 Apr;77(5):517-529. doi: 10.1002/pros.23291. Epub 2017 Jan 17.
Chlamydia trachomatis urogenital infections are the leading cause of sexually transmitted bacterial infections. Although the prevalence of chlamydial infection is similar in men and women, current research is mainly focused on women, neglecting the study of male genital tract infections. We, therefore, investigated Chlamydia infection in the rodent male genital tract.
Male NOD and C57BL/6 mice were inoculated in the meatus urethra with C. muridarum. Bacterial DNA, leukocyte infiltration of male genital tract tissues, pelvic pain, and Chlamydia-specific immune responses were analyzed at different time points.
The inoculation of C. muridarum in the meatus urethra of male mice resulted in an ascending and widely disseminated infection of the male genital tract. C. muridarum remained longer and with the highest bacterial burdens in the prostate, thus showing a special tropism for this organ. Infection caused leukocyte infiltration, mainly composed by neutrophils, and also induced early pelvic pain development that rapidly dropped and resolved as the infection became chronic. Bacterial load and leukocyte infiltration was observed in all prostate lobes, although dorsolateral prostate was the most affected lobe. Interestingly, immune responses induced by both mice strains were characterized by the production of high levels of IL-10 during early stages of the infection, with highest and sustained levels observed in NOD mice, which showed to be less efficient in clearing the infection. Chronic infection of the prostate accompanied by local inflammation and pelvic pain development described herein have important implications for the improvement of the diagnosis and for the design of new efficient therapies. Prostate 77:517-529, 2017. © 2017 Wiley Periodicals, Inc.
沙眼衣原体泌尿生殖系统感染是性传播细菌感染的主要原因。尽管衣原体感染在男性和女性中的患病率相似，但目前的研究主要集中在女性，而忽视了男性生殖道感染的研究。因此，我们研究了啮齿动物雄性生殖道中的衣原体感染情况。
将鼠衣原体接种到雄性NOD和C57BL/6小鼠的尿道开口处。在不同时间点分析细菌DNA、雄性生殖道组织中的白细胞浸润、盆腔疼痛和衣原体特异性免疫反应。
将鼠衣原体接种到雄性小鼠的尿道开口处会导致雄性生殖道发生上行性且广泛传播的感染。鼠衣原体在前列腺中停留的时间更长，细菌载量最高，因此显示出对该器官有特殊的嗜性。感染导致白细胞浸润，主要由中性粒细胞组成，还引发了早期盆腔疼痛，随着感染变为慢性，疼痛迅速减轻并消失。在所有前列腺叶中均观察到细菌载量和白细胞浸润，尽管背外侧前列腺是受影响最严重的叶。有趣的是，两种小鼠品系诱导的免疫反应在感染早期的特征是产生高水平的白细胞介素-10，在NOD小鼠中观察到最高且持续的水平，而NOD小鼠在清除感染方面效率较低。本文所述的前列腺慢性感染伴有局部炎症和盆腔疼痛的发生，对改善诊断和设计新的有效治疗方法具有重要意义。《前列腺》77:517 - 529, 2017。© 2017威利期刊公司。
