Carey Alison J, Cunningham Kelly A, Hafner Louise M, Timms Peter, Beagley Kenneth W
School of Life Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
Immunol Cell Biol. 2009 May-Jun;87(4):337-43. doi: 10.1038/icb.2009.3. Epub 2009 Feb 10.
Chlamydia trachomatis infections have been implicated in problems such as pelvic inflammatory disease and infertility in females. Although there are some studies examining the kinetics of ascending infection, there is limited information on the kinetics of pathology development and cellular infiltrate into the reproductive tissues in relation to the effects of inoculating dose, and a better understanding of these is needed. The murine model of female genital tract Chlamydia muridarum infection is frequently used as a model of human C. trachomatis reproductive tract infection. To investigate the kinetics of ascending genital infection and associated pathology development, female BALB/c mice were intravaginally infected with C. muridarum at doses ranging from 5 x 10(2) to 2.6 x 10(6) inclusion forming units. We found that the inoculating dose affects the course of infection and the ascension of bacteria, with the highest dose ascending rapidly to the oviducts. By comparison, the lowest dose resulted in the greatest bacterial load in the lower reproductive tract. Interestingly, we found that the dose did not significantly affect inflammatory cell infiltrate in the various regions. Overall, this data show the effects of infectious dose on the kinetics of ascending chlamydial infection and associated inflammatory infiltration in BALB/c mice.
沙眼衣原体感染与女性盆腔炎和不孕症等问题有关。尽管有一些研究探讨了上行感染的动力学，但关于病理发展动力学以及与接种剂量影响相关的生殖组织细胞浸润的信息有限，因此需要更好地了解这些情况。雌性生殖道鼠衣原体感染的小鼠模型常被用作人类沙眼衣原体生殖道感染的模型。为了研究上行性生殖道感染及相关病理发展的动力学，将雌性BALB/c小鼠经阴道接种剂量范围为5×10²至2.6×10⁶包涵体形成单位的鼠衣原体。我们发现接种剂量会影响感染进程和细菌的上行，最高剂量的细菌迅速上行至输卵管。相比之下，最低剂量导致下生殖道的细菌载量最大。有趣的是，我们发现剂量对各个区域的炎性细胞浸润没有显著影响。总体而言，这些数据显示了感染剂量对BALB/c小鼠沙眼衣原体上行感染动力学及相关炎性浸润的影响。
