用于靶向头颈鳞状细胞癌细胞和癌症干细胞的自靶向载沙林霉素的DSPE-PEG-甲氨蝶呤纳米胶束。

Self-targeted salinomycin-loaded DSPE-PEG-methotrexate nanomicelles for targeting both head and neck squamous cell carcinoma cancer cells and cancer stem cells.

作者信息

Zhu Minhui, Chen Shicai, Hua Libo, Zhang Caiyun, Chen Mengjie, Chen Donghui, Dong Yinmei, Zhang Yingying, Li Meng, Song Xianmin, Chen Huaiwen, Zheng Hongliang

机构信息

Department of Otolaryngology Head & Neck Surgery, Shanghai Changhai Hospital, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.

Sunlipo Biotech Research Center for Nanomedicine, 3688 Tingwei Road, Shanghai 201507, China.

出版信息

Nanomedicine (Lond). 2017 Feb;12(4):295-315. doi: 10.2217/nnm-2016-0382. Epub 2017 Jan 17.

DOI:10.2217/nnm-2016-0382

PMID:28093940

Abstract

AIM

To target both head and neck squamous cell carcinoma (HNSCC) cells and cancer stem cells (CSCs) by salinomycin-loaded DSPE-PEG-MTX (synthesized using DSPE-PEG2000-NH2 and methotrexate) nanomicelles (M-SAL-MTX).

MATERIALS & METHODS: The characterization, antitumor activity and mechanism of M-SAL-MTX were evaluated.

RESULTS & CONCLUSION: M-SAL-MTX showed enhanced inhibitory effect toward both HNSCC CSCs and non-CSCs compared with a single treatment of methotrexate and salinomycin. In nude mice-bearing HNSCC xenografts, M-SAL-MTX suppressed tumor growth more effectively than other controls including combination of methotrexate and salinomycin. Therefore, M-SAL-MTX may provide a strategy for treating HNSCC by targeting both HNSCC CSCs and HNSCC cells.

摘要

目的

通过负载沙林霉素的DSPE-PEG-MTX（使用DSPE-PEG2000-NH2和甲氨蝶呤合成）纳米胶束（M-SAL-MTX）靶向头颈部鳞状细胞癌（HNSCC）细胞和癌症干细胞（CSC）。

材料与方法

评估了M-SAL-MTX的表征、抗肿瘤活性及作用机制。

结果与结论

与单独使用甲氨蝶呤和沙林霉素治疗相比，M-SAL-MTX对HNSCC CSC和非CSC均表现出增强的抑制作用。在携带HNSCC异种移植瘤的裸鼠中，M-SAL-MTX比包括甲氨蝶呤和沙林霉素联合使用在内的其他对照更有效地抑制肿瘤生长。因此，M-SAL-MTX可能为通过靶向HNSCC CSC和HNSCC细胞来治疗HNSCC提供一种策略。