Key Laboratory of Genetics, Breeding and Comprehensive Utilization of Crops, Ministry of Education, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Unité de Recherche en Génomique Végétale (Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université d'Evry Val d'Essonnes), Organization and Evolution of Plant Genomes, 91057 Evry cedex, France.
Sci Rep. 2017 Jan 17;7:40690. doi: 10.1038/srep40690.
More and more RING finger genes were found to be implicated in various important biological processes. In the present study, a total of 731 RING domains in 715 predicted proteins were identified in Brassica rapa genome (AA, 2n = 20), which were further divided into eight types: RING-H2 (371), RING-HCa (215), RING-HCb (47), RING-v (44), RING-C2 (38), RING-D (10), RING-S/T (5) and RING-G (1). The 715 RING finger proteins were further classified into 51 groups according to the presence of additional domains. 700 RING finger protein genes were mapped to the 10 chromosomes of B. rapa with a range of 47 to 111 genes for each chromosome. 667 RING finger protein genes were expressed in at least one of the six tissues examined, indicating their involvement in various physiological and developmental processes in B. rapa. Hierarchical clustering analysis of RNA-seq data divided them into seven major groups, one of which includes 231 members preferentially expressed in leaf, and constitutes then a panel of gene candidates for studying the genetic and molecular mechanisms of leafy head traits in Brassica crops. Our results lay the foundation for further studies on the classification, evolution and putative functions of RING finger protein genes in Brassica species.
越来越多的环指基因被发现参与各种重要的生物学过程。在本研究中，在芸薹属基因组（AA，2n=20）中鉴定出了总共 731 个 RING 结构域，这些结构域存在于 715 个预测蛋白中，进一步分为 8 种类型：RING-H2（371）、RING-HCa（215）、RING-HCb（47）、RING-v（44）、RING-C2（38）、RING-D（10）、RING-S/T（5）和 RING-G（1）。715 个 RING 指蛋白根据额外结构域的存在进一步分为 51 组。700 个 RING 指蛋白基因被映射到芸薹属的 10 条染色体上，每条染色体的基因数量为 47 到 111 个。667 个 RING 指蛋白基因在至少一种六个检测组织中表达，表明它们参与了芸薹属中各种生理和发育过程。RNA-seq 数据的层次聚类分析将它们分为七个主要组，其中一个组包含 231 个成员，优先在叶片中表达，构成了研究芸薹属作物叶球性状遗传和分子机制的候选基因。我们的研究结果为进一步研究 RING 指蛋白基因在芸薹属物种中的分类、进化和可能功能奠定了基础。
