Chang Jessica, Baker Julie, Wills Andrea
Department of Genetics, Stanford University, Stanford, California, 94305.
Department of Obstetrics and Gynecology, Stanford University, Stanford, California, 94305.
Genesis. 2017 Jan;55(1-2). doi: 10.1002/dvg.23015.
In contrast to humans, many amphibians are able to rapidly and completely regenerate complex tissues, including entire appendages. Following tail amputation, Xenopus tropicalis tadpoles quickly regenerate muscle, spinal cord, cartilage, vasculature and skin, all properly patterned in three dimensions. To better understand the molecular basis of this regenerative competence, we performed a transcriptional analysis of the first 72 h of tail regeneration using RNA-Seq. Our analysis refines the windows during which many key biological signaling processes act in regeneration, including embryonic patterning signals, immune responses, bioelectrical signaling and apoptosis. Our work provides a deep database for researchers interested in appendage regeneration, and points to new avenues for further study.
与人类不同，许多两栖动物能够快速且完全地再生复杂组织，包括整个附肢。在尾部截肢后，热带爪蟾蝌蚪能迅速再生肌肉、脊髓、软骨、脉管系统和皮肤，所有这些组织在三维空间中都能正确地形成图案。为了更好地理解这种再生能力的分子基础，我们使用RNA测序对尾部再生的前72小时进行了转录分析。我们的分析细化了许多关键生物信号传导过程在再生中起作用的时间段，包括胚胎图案化信号、免疫反应、生物电信号传导和细胞凋亡。我们的工作为对附肢再生感兴趣的研究人员提供了一个深入的数据库，并指出了进一步研究的新途径。
