对来自印度的牛疱疹病毒1型分离株糖蛋白B基因序列进行系统发育分析，结果显示1.1亚型占主导地位。
Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1.
作者信息
Patil S S, Prajapati A, Hemadri D, Suresh K P, Desai G S, Reddy G B Manjunatha, Chandranaik B M, Ranganatha S, Rahman H
机构信息
ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru - 560 064, Karnataka, India.
Institute of Animal Health and Veterinary Biologicals, Bengaluru - 560 024, Karnataka, India.
出版信息
Vet World. 2016 Dec;9(12):1364-1369. doi: 10.14202/vetworld.2016.1364-1369. Epub 2016 Dec 5.
AIM
This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease.
MATERIALS AND METHODS
Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software.
RESULTS
A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain.
CONCLUSION
BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain.
目的
本研究旨在从表现出呼吸道疾病临床症状的自然感染牛采集的鼻拭子和阴道拭子中分离牛疱疹病毒1型（BoHV-1）并进行分子特征分析。
材料与方法
对从印度五个邦的65头牛采集的临床样本进行BoHV-1病毒分离。使用针对糖蛋白B（gB）基因组区域的引物通过聚合酶链反应（PCR）进一步确认BoHV-1分离株。使用先前发表的gB基因特异性引物对进行PCR扩增。对从所有分离株获得的gB PCR扩增子进行测序，并使用软件进行系统发育分析。
结果
在细胞培养分离中总共发现12个样本呈阳性。11个分离株在72小时后对Madin-Darby牛肾细胞显示出明显的细胞病变效应。所有分离株gB基因的部分序列分析显示它们之间的同源性为99.0 - 100%。所有分离株与库珀毒株显示出99.2 - 99.8%的同源性。
结论
BoHV-1.1是印度BoHV的主要流行亚型，并且所有分离株与库珀毒株具有同源性。
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