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极低频电磁场对……影响的评估

Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on .

作者信息

Ozlem-Caliskan Sercin, Ertabaklar Hatice, Bilgin Mehmet Dincer, Ertug Sema

机构信息

Department of Biophysics, Institute of Health Sciences, Adnan Menderes University, Aydin, Turkey.

Department of Parasitology, Medical Faculty, Adnan Menderes University, Aydin, Turkey.

出版信息

Iran J Parasitol. 2016 Apr-Jun;11(2):159-167.

Abstract

BACKGROUND

The effects of extremely low frequency electromagnetic fields (ELF-EMF) on have not been explained yet. The aim of this study was to assess the possible effects of ELF-EMF on growth, survival time and viability of . In addition, the life span of infected animals was investigated.

METHODS

Sixty adult male BALB/c mice were used for in vivo and in vivo experiments in Laboratory of Biopyhsics and Parasitology of Medical Faculty, Adnan Menderes University, Turkey, in 2010. During in vivo experiments, pulsed and continuous EMFs were applied for 5 d to the infected mice. During in vivo experiments, pulsed and continuous EMF was applied to the tachyzoites within peritoneal exudates for 8 h/d at 4 °C and the tachyzoites were then injected to mice. In both experiments, the number of in peritoneal exudates was counted and protein bands patterns were investigated with polyacrylamide gel electrophoresis and Western Blotting.

RESULTS

Pulsed and continuous EMF exposure reduced the number of tachyzoites in comparison to controls. However, no statistically significant differences were observed at the patterns of protein bands among the samples.

CONCLUSION

EMF exposure induces a decrease in the number of . Further studies are required to understand the mechanism of EMF on intracellular parasites.

摘要

背景

极低频电磁场(ELF - EMF)对[具体对象未提及]的影响尚未得到解释。本研究的目的是评估ELF - EMF对[具体对象未提及]的生长、存活时间和活力的可能影响。此外,还研究了感染动物的寿命。

方法

2010年,在土耳其阿德南·曼德列斯大学医学院生物物理学与寄生虫学实验室,使用60只成年雄性BALB/c小鼠进行体内和体外实验。在体内实验中,对感染小鼠施加脉冲和连续电磁场5天。在体外实验中,将脉冲和连续电磁场在4℃下对腹腔渗出液中的速殖子施加8小时/天,然后将速殖子注射到小鼠体内。在两个实验中,对腹腔渗出液中的[具体对象未提及]数量进行计数,并用聚丙烯酰胺凝胶电泳和蛋白质印迹法研究[具体对象未提及]蛋白质条带模式。

结果

与对照组相比,脉冲和连续电磁场暴露减少了[具体对象未提及]速殖子的数量。然而,在样本间蛋白质条带模式上未观察到统计学上的显著差异。

结论

电磁场暴露导致[具体对象未提及]数量减少。需要进一步研究以了解电磁场对细胞内寄生虫的作用机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e662/5236092/23902d68a017/IJPA-11-159-g001.jpg

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