Stenkula Karin G, Stenblom Eva-Lena, Montelius Caroline, Egecioglu Emil, Erlanson-Albertsson Charlotte
Glucose Transport and Protein Trafficking, Department of Experimental Medical Science, BMC, Lund University, 221 84 Lund, Sweden.
Appetite Control, Department of Experimental Medical Science, BMC, Lund University, 221 84 Lund, Sweden.
Nutr Metab (Lond). 2017 Jan 11;14:4. doi: 10.1186/s12986-016-0160-4. eCollection 2017.
Dietary thylakoids derived from spinach have beneficial effects on body fat accumulation and blood lipids as demonstrated in humans and rodents. Important mechanisms established include delayed fat digestion in the intestine, without causing steatorrhea, and increased fatty acid oxidation in intestinal cells. The objective of our study was to elucidate if increased fecal fat excretion is an important mechanism to normalize adipose tissue metabolism during high-fat feeding in mice supplemented with thylakoids.
Mice were randomized to receive HFD or thylHFD for 14 days ( = 14 for the control group and 16 for the thylakoid group). The effect of thylakoids on body fat distribution, faecal and liver fat content, and adipose tissue metabolism was investigated following high-fat feeding.
Thylakoid supplementation for 14 days caused an increased faecal fat content without compensatory eating compared to control. As a result, thylakoid treated animals had reduced fat mass depots and reduced liver fat accumulation compared to control. The size distribution of adipocytes isolated from visceral adipose tissue was narrowed and the cell size decreased. Adipocytes isolated from thylakoid-treated mice displayed a significantly increased lipogenesis, and protein expression of peroxisome proliferator-activated receptor gamma (PPARγ), down-stream target FAS, as well as transcription factor coactivators PGC1-α and LPIN-1 were upregulated in adipose tissue from thylakoid-fed mice.
Together, these data suggest that thylakoid supplementation reduces body fat and fat cell size by binding to dietary fat and increasing its fecal excretion, thus reducing dietary fat available for absorption.
来源于菠菜的膳食类囊体对人体和啮齿动物的体脂积累和血脂具有有益作用。已确立的重要机制包括肠道内脂肪消化延迟且不引起脂肪泻，以及肠道细胞中脂肪酸氧化增加。我们研究的目的是阐明粪便脂肪排泄增加是否是补充类囊体的小鼠在高脂喂养期间使脂肪组织代谢正常化的重要机制。
将小鼠随机分为接受高脂饮食（HFD）或类囊体高脂饮食（thylHFD）14天（对照组为14只，类囊体组为16只）。在高脂喂养后，研究类囊体对体脂分布、粪便和肝脏脂肪含量以及脂肪组织代谢的影响。
与对照组相比，补充类囊体14天导致粪便脂肪含量增加且无代偿性进食。结果，与对照组相比，接受类囊体处理的动物脂肪量储存减少，肝脏脂肪积累减少。从内脏脂肪组织分离的脂肪细胞大小分布变窄，细胞大小减小。从接受类囊体处理的小鼠分离的脂肪细胞显示脂肪生成显著增加，并且在接受类囊体喂养的小鼠的脂肪组织中，过氧化物酶体增殖物激活受体γ（PPARγ）、下游靶点脂肪酸合酶（FAS）以及转录因子共激活因子PGC1-α和LPIN-1的蛋白表达上调。
总之，这些数据表明补充类囊体通过与膳食脂肪结合并增加其粪便排泄来减少体脂和脂肪细胞大小，从而减少可供吸收的膳食脂肪。
