Yang Yuangui, Zhang Ji, Jin Hang, Zhang Jinyu, Wang Yuanzhong
College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China.
J Anal Methods Chem. 2016;2016:3182796. doi: 10.1155/2016/3182796. Epub 2016 Dec 21.
A fast method was developed by ultra high performance liquid chromatography (UHPLC) for simultaneous determination of polyphyllin I and polyphyllin II. Chemometric analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) based on UHPLC chromatography were used to evaluate 38 batches from six species of . Variable importance of projection was applied to select important peaks. Meanwhile, similarity analysis of UHPLC fingerprint was used to evaluate the sample of (PPY) and (PA). The results indicated that the total content of saponins in PPY and PA collected from Baoshan City of Yunnan Province above 8.07 mg/g was stronger than that from other areas of the rest of species. PLS-DA showed better performance than PCA with regard to classifying the samples. Retention time during 20-27 minutes of UHPLC was screened as significant peak for distinguishing of different species and original geography. All of PPY and PA with similarity value were more than 0.80. It indicated that quantitative analysis combined with chemometric and similarity analyses could evaluate the different species of effectively and comprehensively.
建立了一种超高效液相色谱（UHPLC）快速同时测定重楼皂苷I和重楼皂苷II的方法。基于UHPLC色谱的化学计量学分析，包括主成分分析（PCA）和偏最小二乘判别分析（PLS-DA），用于评价6种重楼属植物的38个批次。采用变量投影重要性选择重要峰。同时，利用UHPLC指纹图谱相似度分析对滇重楼（PPY）和七叶一枝花（PA）样品进行评价。结果表明，云南省保山市采集的滇重楼和七叶一枝花中皂苷总含量高于8.07 mg/g，高于其他地区的其他种。在样本分类方面，PLS-DA表现优于PCA。筛选出UHPLC在20-27分钟的保留时间作为区分不同种和原生产地的重楼属植物的显著峰。所有滇重楼和七叶一枝花的相似度值均大于0.80。表明定量分析结合化学计量学和相似度分析能够有效、全面地评价不同种的重楼属植物。
