Yang Yuangui, Jin Hang, Zhang Ji, Zhang Jinyu, Wang Yuanzhong
College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China.
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.
J Nat Med. 2017 Jan;71(1):148-157. doi: 10.1007/s11418-016-1044-7. Epub 2016 Sep 24.
Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz (PPY) is used widely as an anthelmintic, antimicrobial, and anti-tumor agent. Multiplicate analytical methods have been employed to discriminate PPY from different regions, as well as to identify regions most beneficial to the growing of this species. In this study, a convenient and accurate method was established using ultra high performance liquid chromatography (UHPLC) for simultaneous determination of four steroid saponins (Pa, Pb, polyphyllin VI, and chonglou saponin VII). Partial least squares discriminant analysis (PLS-DA) according to UHPLC and UV spectroscopy was applied to analyze 30 samples of PPY from three regions of Yunnan Province in China, and identify significant peaks. The results indicated that the correlation coefficients (r ) of all calibration curves were above 0.999, and the inter- and intra-day relative standard deviations (RSD) of retention time and peak areas of common peaks were below 1.78 % and 3.40 %, respectively, with recovery rates of 99.6-103.4 % with RSD ≤2 %. Quantitative analysis implied that the average values of total saponins in PPY from south Yunnan Province (19.9 mg/g) were higher than in the central (8.79 mg/g) district. Thus, further investigation could focus on the southern region to seek high quality PPY. The analysis found that PLS-DA for ultraviolet (UV) spectroscopy, which could separate the samples from three regions, was more appropriate than UHPLC. Retention times during 20-30.75 min of UHPLC, and absorption at 200-300 nm of the UV spectrum were identified as significant peaks for distinguishing PPY from different regions.
滇重楼（Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz，PPY）被广泛用作驱虫剂、抗菌剂和抗肿瘤剂。人们采用了多种分析方法来区分不同产地的滇重楼，并确定最有利于该物种生长的区域。在本研究中，建立了一种简便准确的超高效液相色谱（UHPLC）法，用于同时测定四种甾体皂苷（Pa、Pb、重楼皂苷VI和重楼皂苷VII）。根据UHPLC和紫外光谱进行偏最小二乘判别分析（PLS-DA），以分析来自中国云南省三个地区的30份滇重楼样品，并识别出显著峰。结果表明，所有校准曲线的相关系数（r）均高于0.999，保留时间和共有峰峰面积的日内和日间相对标准偏差（RSD）分别低于1.78%和3.40%，回收率为99.6 - 103.4%，RSD≤2%。定量分析表明，滇南地区滇重楼总皂苷的平均值（19.9 mg/g）高于中部地区（8.79 mg/g）。因此，进一步的研究可以集中在南部地区，以寻找高质量的滇重楼。分析发现，用于紫外（UV）光谱的PLS-DA比UHPLC更适合分离来自三个地区的样品。UHPLC在20 - 30.75分钟的保留时间以及UV光谱在200 - 300 nm处的吸收被确定为区分不同地区滇重楼的显著峰。
