Yang Won-Kyung, Lee Chul-Hwa, Kim Min-Hee, Kim Seung-Hyeong, Choi Hae-Yoon, Yeo Yoon, Park Yang-Chun
Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon, Korea.
Department of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University, Daejeon, Korea.
J Pharmacopuncture. 2016 Dec;19(4):303-311. doi: 10.3831/KPI.2016.19.031.
Allergic asthma generally presents with symptoms of wheezing, coughing, breathlessness, and airway inflammation. Seonpyejeongcheon-tang (SJT) consists of 12 herbs. It originated from Jeongcheon-tang (JT), also known as Ding-chuan-tang, composed of 7 herbs, in She-sheng-zhong-miao-fang. This study aimed to evaluate the effects of local delivery of SJT inhalable microparticles in an asthma mouse model.
Microparticles containing SJT were produced by spray-drying with leucine as an excipient. SJT microparticles were evaluated with respect to their aerodynamic properties, cytotoxicity, toxicity, and therapeutic effects on ovalbumin (OVA)-induced asthma in comparison with orally-administered SJT.
SJT microparticles provided desirable aerodynamic properties (fine particle fraction of 48.9% ± 6.4% and mass median aerodynamic diameter of 3.7 ± 0.3 μm). SJT microparticles did not show any cytotoxicity against RAW 264.7 macrophages at concentrations of 0.01 - 3 mg/mL. Inhaled SJT microparticles decreased the levels of IL-4, IL-5, IL-13, IL-17A, eotaxin and OVA-IgE in bronchoalveolar lavage fluid (BALF) in mice with OVA-induced asthma. These effects were verified by histological evaluation of the levels of infiltration of inflammatory cells and collagen, destructions of alveoli and bronchioles, and hyperplasia of goblet cells in lung tissues. The effects of SJT microparticles in the asthma model were equivalent to those of orally-administered SJT extract.
This study suggests that SJT is a promising agent for inhalation therapy for patients with asthma.
过敏性哮喘通常表现为喘息、咳嗽、呼吸急促和气道炎症等症状。仙脾定喘汤（SJT）由12味草药组成。它源自《摄生众妙方》中的定喘汤（JT，又称定喘汤），定喘汤由7味草药组成。本研究旨在评估SJT可吸入微粒局部给药对哮喘小鼠模型的影响。
以亮氨酸为辅料，通过喷雾干燥法制备含SJT的微粒。与口服SJT相比，对SJT微粒的空气动力学性质、细胞毒性、毒性以及对卵清蛋白（OVA）诱导的哮喘的治疗效果进行评估。
SJT微粒具有理想的空气动力学性质（细颗粒分数为48.9%±6.4%，质量中值空气动力学直径为3.7±0.3μm）。在浓度为0.01 - 3mg/mL时，SJT微粒对RAW 264.7巨噬细胞未表现出任何细胞毒性。吸入SJT微粒可降低OVA诱导的哮喘小鼠支气管肺泡灌洗液（BALF）中IL-4、IL-5、IL-13、IL-17A、嗜酸性粒细胞趋化因子和OVA-IgE的水平。通过对肺组织中炎性细胞浸润、胶原蛋白水平、肺泡和细支气管破坏以及杯状细胞增生的组织学评估，验证了这些效果。SJT微粒在哮喘模型中的作用与口服SJT提取物的作用相当。
本研究表明，SJT是一种有前景的用于哮喘患者吸入治疗的药物。
