Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 710069, China.
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 710069, China.
J Ethnopharmacol. 2017 Jul 31;207:34-41. doi: 10.1016/j.jep.2017.06.016. Epub 2017 Jun 15.
Pulvis Fellis Suis (PFS), named with "Zhu Danfen" in China, has been extensively used for the therapy of enteritis, acute pharyngitis, whooping cough and asthma in folk medicine. Although PFS shows anti-inflammatory activities, its effect on airway inflammation in asthma has not been studied.
To explore the protective effect of PFS ethanol extract against airway inflammation in asthmatic mice.
Allergic asthma in mice was sensitized and challenged by OVA. Mice were administered in oral with PFS daily at doses of 100, 200 and 400mg/kg on days 21-27. Inflammatory cell counts and classification in bronchoalveolar lavage fluid (BALF) were analyzed. Histopathological evaluation of the lung tissue was performed by hematoxylin and eosin (H&E) and periodic acid-schiff (PAS) staining. The IgE level in serum was measured by using enzyme-linked immunoassay (ELISA). ELISA was also used to detect the levels of Th1/Th2 cytokine and eotaxin in BALF.
Histological results revealed that PFS could ameliorate OVA-induced histological changes by attenuating inflammatory cell infiltration, mucus hypersecretion and goblet cell hyperplasia in the lung. Treatment with different doses of PFS significantly decreased the elevated inflammatory cell numbers in BALF and IgE production in serum. PFS treatment reduced the production of Th2 cytokine IL-4, IL-5, IL-13, and promoted Th1 cytokine IFN-γ production in BALF. In addition, PFS also decreased the levels of eotaxin and TNF-α in BALF.
These findings suggest that PFS has a markedly anti-inflammatory effect on OVA-induced allergic asthma in mice, and could be a promising protective agent recommended for allergic asthma patients.
在中国被命名为“猪胆粉”的猪胆粉（PFS），在民间医学中被广泛用于治疗肠炎、急性咽炎、百日咳和哮喘。尽管 PFS 表现出抗炎活性，但尚未研究其对哮喘气道炎症的影响。
探讨 PFS 乙醇提取物对哮喘小鼠气道炎症的保护作用。
通过 OVA 致敏和激发建立变应性哮喘小鼠模型。在第 21-27 天，每天通过口服给予 PFS 治疗，剂量分别为 100、200 和 400mg/kg。分析支气管肺泡灌洗液（BALF）中炎性细胞计数和分类。通过苏木精和伊红（H&E）和过碘酸希夫（PAS）染色对肺组织进行组织病理学评估。通过酶联免疫吸附试验（ELISA）测定血清 IgE 水平。ELISA 还用于检测 BALF 中 Th1/Th2 细胞因子和嗜酸性粒细胞趋化因子（eotaxin）的水平。
组织学结果表明，PFS 通过减轻肺部炎症细胞浸润、黏液分泌过度和杯状细胞增生，可改善 OVA 诱导的组织学变化。用不同剂量的 PFS 治疗可显著降低 BALF 中升高的炎性细胞数和血清 IgE 产生。PFS 治疗降低了 BALF 中 Th2 细胞因子 IL-4、IL-5、IL-13 的产生，并促进了 Th1 细胞因子 IFN-γ的产生。此外，PFS 还降低了 BALF 中 eotaxin 和 TNF-α的水平。
这些发现表明，PFS 对 OVA 诱导的哮喘小鼠具有明显的抗炎作用，可能是一种有前途的过敏性哮喘保护剂。
