Chen Qicong, Ma Chengtong, Qian Jieying, Lan Xiuwan, Chao Naixia, Sun Jian, Wu Yaosheng
Key Laboratory of Biological Molecular Medicine Research of Guangxi Higher Education, Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning, Guangxi, China.
Int J Genomics. 2016;2016:7840914. doi: 10.1155/2016/7840914. Epub 2016 Dec 14.
(), a creeping herbaceous perennial with many important medicinal properties, is widely distributed in Asia. Gypenosides (triterpenoid saponins), the main effective components of , are well studied. FPS (farnesyl pyrophosphate synthase), SS (squalene synthase), and SE (squalene epoxidase) are the main enzymes involved in the synthesis of triterpenoid saponins. Considering the important medicinal functions of , it is necessary to investigate the transcriptomic information of to facilitate future studies of transcriptional regulation. After sequencing , we obtained 50,654,708 unigenes. Next, we used RPKM (reads per kilobases per million reads) to calculate expression of the unigenes and we performed comparison of our data to that contained in five common databases to annotate different aspects of the unigenes. Finally, we noticed that FPS, SS, and SE showed differential expression of enzymes in DESeq. Leaves showed the highest expression of FPS, SS, and SE relative to the other two tissues. Our research provides transcriptomic information of in its natural environment and we found consistency in unigene expression, enzymes expression (FPS, SS, and SE), and the distribution of gypenosides content in . Our results will enable future related studies of .
（绞股蓝），一种具有多种重要药用特性的多年生匍匐草本植物，在亚洲广泛分布。绞股蓝皂苷（三萜皂苷）作为绞股蓝的主要有效成分，已得到充分研究。法尼基焦磷酸合酶（FPS）、鲨烯合酶（SS）和鲨烯环氧酶（SE）是参与三萜皂苷合成的主要酶。鉴于绞股蓝的重要药用功能，有必要研究其转录组信息以促进未来转录调控研究。对绞股蓝进行测序后，我们获得了50,654,708个单基因。接下来，我们使用每百万 reads 中每千碱基的 reads 数（RPKM）来计算单基因的表达量，并将我们的数据与五个常用数据库中的数据进行比较，以注释单基因的不同方面。最后，我们注意到在DESeq中FPS、SS和SE显示出酶的差异表达。相对于其他两个组织，叶片中FPS、SS和SE的表达最高。我们的研究提供了绞股蓝在自然环境中的转录组信息，并且我们发现绞股蓝中基因表达、酶表达（FPS、SS和SE）以及绞股蓝皂苷含量分布具有一致性。我们的结果将有助于未来对绞股蓝的相关研究。
