Khandare Rahul V, Desai Shaileshkumar B, Bhujbal Sourabh S, Watharkar Anuprita D, Biradar Shivtej P, Pawar Pankaj K, Govindwar Sanjay P
Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, 416004, India.
Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur, 416004, India.
Environ Sci Pollut Res Int. 2017 Mar;24(7):6833-6839. doi: 10.1007/s11356-017-8424-8. Epub 2017 Jan 17.
Nursery grown plants of Nerium oleander, Pogonatherum crinitum, and Portulaca oleracea were observed to remove fluoride up to 92, 80, and 73%, respectively, from NaF solution at the concentration of 10 mg L within 15 days. Concentration range of 10-50 mg L of fluoride revealed a constant decrease of removal from 92 to 51% within 15 days by N. oleander, while the biomass (one to five plants) showed enhancement in removal from 74 to 98% in 10 days. Translocation and bioaccumulation factors calculated after fluoride contents in roots and leaves of N. oleander, P. crinitum, and P. oleracea were 1.85, 1.19, and 1.43, and 9.8, 3.6, and 2.2, respectively. P . oleracea, P. crinitum, and N. oleander showed reductions in chlorophyll contents by 40, 57 and 25 and 8%, carbohydrates by 50, 44, and 16%, and proteins by 38, 53, and 15%, respectively. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in the roots of P. oleracea, P. crinitum, and N. oleander were observed to be induced by 400, 383, and 500%; 80, 105, and 424%; and 153, 77, and 71%, respectively, while the leaves showed induction in SOD, CAT, and GPX activities by 550, 315, and 165%; 196, 227, and 243%; and 280, 242, and 184%, respectively. Results endorsed the superiority of N. oleander for fluoride removal over other plant species.
观察到，在15天内，苗圃培育的夹竹桃、粽叶芦和马齿苋分别能从浓度为10毫克/升的氟化钠溶液中去除高达92%、80%和73%的氟化物。在10 - 50毫克/升的氟化物浓度范围内，夹竹桃在15天内的去除率从92%持续下降至51%，而生物量（1 - 5株植物）在10天内的去除率从74%提高至98%。夹竹桃、粽叶芦和马齿苋根与叶中的氟化物含量经计算后，其转运因子分别为1.85、1.19和1.43，生物积累因子分别为9.8、3.6和2.2。马齿苋、粽叶芦和夹竹桃的叶绿素含量分别降低了40%、57%、25%和8%，碳水化合物含量分别降低了50%、44%和16%，蛋白质含量分别降低了38%、53%和15%。观察到马齿苋、粽叶芦和夹竹桃根中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）活性分别被诱导提高了400%、383%和500%；80%、105%和424%；153%、77%和71%，而叶中的SOD、CAT和GPX活性分别被诱导提高了550%、315%和165%；196%、227%和243%；280%、242%和184%。结果证实了夹竹桃在去除氟化物方面优于其他植物物种。
