Pepper Victoria K, Clark Elizabeth S, Best Cameron A, Onwuka Ekene A, Sugiura Tadahisa, Heuer Eric D, Moko Lilamarie E, Miyamoto Shinka, Miyachi Hideki, Berman Darren P, Cheatham Sharon L, Chisolm Joanne L, Shinoka Toshiharu, Breuer Christopher K, Cheatham John P
Tissue Engineering Program, Research Institute at Nationwide Children's Hospital, 700 Children's Drive, WB 4154, Columbus, OH, 43205, USA.
Department of Pediatric Surgery, Nationwide Children's Hospital, Columbus, OH, USA.
J Cardiovasc Transl Res. 2017 Apr;10(2):128-138. doi: 10.1007/s12265-016-9725-x. Epub 2017 Jan 17.
Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model. Eight sheep underwent implantation of TEVGs either unseeded or seeded with bone marrow-derived mononuclear cells. Angiography, IVUS, and histology were directly compared. Endothelium, tunica media, and graft were identifiable on IVUS and histology at multiple time points. There was strong agreement between IVUS and angiography for evaluation of luminal diameter. IVUS offers a valuable tool to evaluate the changes within TEVGs, and clinical translation of this application is warranted.
接受组织工程血管移植物（TEVG）植入治疗先天性心脏异常的患者通过超声心动图进行监测，必要时随后进行磁共振成像或血管造影。虽然这些方法可提供有关管腔的数据，但关于新组织形成的信息极少。血管内超声（IVUS）此前已用于多种情况以评估血管壁。本研究的目的是评估IVUS在我们的绵羊模型中评估TEVG的效用。八只绵羊接受了未接种或接种骨髓来源单核细胞的TEVG植入。对血管造影、IVUS和组织学进行了直接比较。在多个时间点，IVUS和组织学上均可识别内皮、中膜和移植物。IVUS与血管造影在评估管腔直径方面具有高度一致性。IVUS为评估TEVG内的变化提供了一种有价值的工具，这种应用的临床转化是有必要的。
