Platt-Aloia K A, Thomson W W
Department of Botany and Plant Sciences, University of California, Riverside 92521.
J Electron Microsc Tech. 1989 Dec;13(4):288-99. doi: 10.1002/jemt.1060130404.
The use of whole, intact plant tissue for freeze-fracture electron microscopy provides important information that cannot be obtained from the use of isolated biological membranes or of artificial (phospholipid) membrane preparations. This is not to imply that these examinations of such preparations are not useful, since it would be difficult to interpret our observations of intact cells and tissues without the analysis of these model systems. Analysis of intact tissue and cells reveals the relative densities of membrane proteins of the different membranes within a cell; the three-dimensional organization of various organelles, especially the endoplasmic reticulum; changes in intramembranous particle (IMP) distribution due to stress or injury; and, in conjunction with the use of filipin, membrane sterol content and relative distribution. It is our intention that this survey of freeze-fracture images of intact plant tissues will illustrate the uniqueness of the information gained from an analysis of whole plant tissues compared to isolated membrane fractions.
使用完整的植物组织进行冷冻断裂电子显微镜检查可提供重要信息，而这些信息无法通过使用分离的生物膜或人工（磷脂）膜制剂获得。这并不是说对这些制剂的检查没有用处，因为如果不分析这些模型系统，就很难解释我们对完整细胞和组织的观察结果。对完整组织和细胞的分析揭示了细胞内不同膜的膜蛋白相对密度；各种细胞器，特别是内质网的三维组织结构；由于压力或损伤导致的膜内颗粒（IMP）分布变化；以及结合使用制霉菌素时的膜固醇含量和相对分布。我们的目的是，通过对完整植物组织冷冻断裂图像的调查，说明与分离的膜组分相比，分析整个植物组织所获得信息的独特性。
