Chailley B, Boisvieux-Ulrich E, Sandoz D
J Submicrosc Cytol. 1983 Jan;15(1):275-80.
Membrane events during ciliogenesis were studied in the quail oviduct cells. Two membrane domains differentiate during ciliogenesis: the ciliary necklace progressively composed of one to six rows of intramembranous particle (IMPs), and the shaft membrane poor in IMPs but characterized by a high content of sterol. Membrane sterol can be detected by the use of filipin which forms filipin-sterol complexes (f-c complexes) visible in electron microscopy on both freeze fracture replicas and thin sections. During ciliary growth, the necklace IMP rows are progressively organized in an area completely devoid of f-c complexes whereas the membrane of growing shaft shows a high density of f-c complexes except at the ciliary tip.
在鹌鹑输卵管细胞中研究了纤毛发生过程中的膜事件。在纤毛发生过程中，两个膜结构域发生分化：纤毛项链逐渐由一到六排膜内颗粒（IMP）组成，而轴膜中IMP较少，但以高含量的固醇为特征。膜固醇可以通过使用制霉菌素检测，制霉菌素会形成制霉菌素 - 固醇复合物（f-c复合物），在冷冻断裂复制品和超薄切片的电子显微镜下都可见。在纤毛生长过程中，项链IMP排逐渐在一个完全没有f-c复合物的区域组织起来，而正在生长的轴膜除了在纤毛尖端外，显示出高密度的f-c复合物。
